首页> 美国卫生研究院文献>Scientific Reports >Response surface optimization of culture medium for enhanced docosahexaenoic acid production by a Malaysian thraustochytrid
【2h】

Response surface optimization of culture medium for enhanced docosahexaenoic acid production by a Malaysian thraustochytrid

机译:培养基的响应面优化以提高马来西亚破囊壶菌产生二十二碳六烯酸的能力

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Docosahexaenoic acid (DHA, C22:6n-3) plays a vital role in the enhancement of human health, particularly for cognitive, neurological, and visual functions. Marine microalgae, such as members of the genus Aurantiochytrium, are rich in DHA and represent a promising source of omega-3 fatty acids. In this study, levels of glucose, yeast extract, sodium glutamate and sea salt were optimized for enhanced lipid and DHA production by a Malaysian isolate of thraustochytrid, Aurantiochytrium sp. SW1, using response surface methodology (RSM). The optimized medium contained 60 g/L glucose, 2 g/L yeast extract, 24 g/L sodium glutamate and 6 g/L sea salt. This combination produced 17.8 g/L biomass containing 53.9% lipid (9.6 g/L) which contained 44.07% DHA (4.23 g/L). The optimized medium was used in a scale-up run, where a 5 L bench-top bioreactor was employed to verify the applicability of the medium at larger scale. This produced 24.46 g/L biomass containing 38.43% lipid (9.4 g/L), of which 47.87% was DHA (4.5 g/L). The total amount of DHA produced was 25% higher than that produced in the original medium prior to optimization. This result suggests that Aurantiochytrium sp. SW1 could be developed for industrial application as a commercial DHA-producing microorganism.
机译:二十二碳六烯酸(DHA,C22:6n-3)在增强人类健康方面,尤其是在认知,神经和视觉功能方面,起着至关重要的作用。海洋微藻,例如Aurantiochytrium属的成员,富含DHA,并代表了有希望的omega-3脂肪酸来源。在这项研究中,通过马来西亚破囊壶菌(Aurantiochytrium sp)的分离株,优化了葡萄糖,酵母提取物,谷氨酸钠和海盐的水平,以增强脂质和DHA的产生。 SW1,使用响应面方法(RSM)。优化后的培养基包含60μg/ L葡萄糖,2μg/ L酵母提取物,24μg/ L谷氨酸钠和6μg/ L海盐。该组合产生了17.8μg/ L的生物质,其包含53.9%的脂质(9.6μg/ L),其包含44.07%的DHA(4.23μg/ L)。优化的培养基用于放大实验,其中使用5 µL台式生物反应器验证该培养基在更大规模上的适用性。这产生了24.46μg/ L的生物质,其包含38.43%的脂质(9.4μg/ L),其中47.87%是DHA(4.5μg/ L)。优化产生的DHA总量比原始培养基中产生的DHA高25%。该结果表明Aurantiochytrium sp。可以将SW1开发为工业用途的商业DHA生产微生物。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号