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首页> 美国卫生研究院文献>Cancer Science >Expression of Drug Resistance‐related Genes in Head and Neck Squamous Cell Carcinoma and Normal Mucosa
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Expression of Drug Resistance‐related Genes in Head and Neck Squamous Cell Carcinoma and Normal Mucosa

机译：耐药相关基因在头颈部鳞状细胞癌和正常黏膜中的表达

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We examined the expression levels of mRNA for multidrug resistance 1 (MDR1), multidrug resistance‐associated protein (MRP), human canalicular multispecific organic anion transporter (cMOAT), lung resistance‐related protein (LRP), topoisomerase IIα, β(Topo IIα, β) and topoisomerase I (Topo I) genes in human head and neck squamous cell carcinoma (HNSCC) specimens and mucosa (HNM) specimens, to elucidate their roles in relation to the biological characteristics and drug resistance in vivo. Fifty‐eight samples (45 head and neck carcinomas and 13 head and neck mucosa) obtained during surgical resection or biopsy from 38 patients were analyzed using the quantitative reverse transcription‐polymerase chain reaction (RT‐PCR) method. MDR1, MRP, LRP, Topo IIα, Topo IIβ, and Topo I gene transcripts were detected in all the samples tested, but cMOAT mRNA was not detected in them. Comparisons of the expression levels in HNSCC with those in HNM showed that the Topo IIα gene expression level was higher in HNSCC than in HNM (P=0.0298). Moreover, the Topo IIα mRNA level was significantly higher in metastatic lymph node samples of HNSCC than in HNM samples (P=0.0205). There were no significant differences in the six genes' expression levels between samples exposed to platinum drugs and those not exposed to platinum drugs. These results suggest that it may be effective in anticancer therapy to use topoisomerase‐targetting drugs against HNSCC, especially metastatic neck tumors, and that the expression of these genes in HNSCC is not associated with platinum drug exposure.
机译：我们检查了多药抗性1（MDR1），多药抗性相关蛋白（MRP），人管多特异性有机阴离子转运蛋白（cMOAT），肺部抗性相关蛋白（LRP），拓扑异构酶IIα，β（TopoIIα）的mRNA表达水平，β）和拓扑异构酶I（Topo I）基因在人类头颈部鳞状细胞癌（HNSCC）标本和粘膜（HNM）标本中的表达，以阐明它们在体内生物学特性和耐药性方面的作用。使用定量逆转录聚合酶链反应（RT-PCR）方法分析了38例患者在手术切除或活检期间获得的58份样本（45例头颈癌和13例头颈黏膜）。在所有测试样品中均检测到MDR1，MRP，LRP，TopoIIα，TopoIIβ和Topo I基因转录本，但未检测到cMOAT mRNA。对HNSCC和HNM中的表达水平进行比较表明，TopoIIα基因在HNSCC中的表达水平高于HNM（P = 0.0298）。此外，HNSCC转移淋巴结样本中的TopoIIαmRNA水平显着高于HNM样本（P = 0.0205）。暴露于铂类药物的样本与未暴露于铂类药物的样本之间的六个基因表达水平无显着差异。这些结果表明，使用针对HNSCC的拓扑异构酶靶向药物（尤其是转移性颈部肿瘤）在抗癌治疗中可能是有效的，并且这些基因在HNSCC中的表达与铂类药物暴露无关。

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