• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Japanese Journal of Cancer Research >Expression of drug resistance-related genes in head and neck squamous cell carcinomas and normal mucosa.
【24h】

Expression of drug resistance-related genes in head and neck squamous cell carcinomas and normal mucosa.

机译：耐药相关基因在头颈部鳞状细胞癌和正常黏膜中的表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We examined the expression levels of mRNA for multidrug resistance 1 (MDR1), multidrug resistance-associated protein (MRP), human canalicular multispecific organic anion transporter (cMOAT), lung resistance-related protein (LRP), topoisomerase IIalpha, beta (Topo IIalpha, beta) and topoisomerase I (Topo I) genes in human head and neck squamous cell carcinoma (HNSCC) specimens and mucosa (HNM) specimens, to elucidate their roles in relation to the biological characteristics and drug resistance in vivo. Fifty-eight samples (45 head and neck carcinomas and 13 head and neck mucosa) obtained during surgical resection or biopsy from 38 patients were analyzed using the quantitative reverse transcription-polymerase chain reaction (RT-PCR) method. MDR1, MRP, LRP, Topo IIalpha, Topo IIbeta, and Topo I gene transcripts were detected in all the samples tested, but cMOAT mRNA was not detected in them. Comparisons of the expression levels in HNSCC with those in HNM showed that the Topo IIalpha gene expression level was higher in HNSCC than in HNM (P=0.0298). Moreover, the Topo IIalpha mRNA level was significantly higher in metastatic lymph node samples of HNSCC than in HNM samples (P=0.0205). There were no significant differences in the six genes' expression levels between samples exposed to platinum drugs and those not exposed to platinum drugs. These results suggest that it may be effective in anticancer therapy to use topoisomerase-targetting drugs against HNSCC, especially metastatic neck tumors, and that the expression of these genes in HNSCC is not associated with platinum drug exposure.
机译：我们检查了多重耐药1（MDR1），多重耐药相关蛋白（MRP），人管多特异性有机阴离子转运蛋白（cMOAT），肺耐药相关蛋白（LRP），拓扑异构酶IIalpha，beta（Topo IIalpha）的mRNA表达水平（beta）和拓扑异构酶I（Topo I）基因在人类头颈部鳞状细胞癌（HNSCC）标本和黏膜（HNM）标本中的表达，以阐明它们在体内生物学特性和耐药性方面的作用。使用定量逆转录-聚合酶链反应（RT-PCR）方法分析了38例患者在手术切除或活检期间获得的58份样本（45例头颈癌和13例头颈黏膜）。在所有测试样品中均检测到MDR1，MRP，LRP，Topo IIalpha，Topo IIbeta和Topo I基因转录本，但未检测到cMOAT mRNA。 HNSCC和HNM中表达水平的比较表明，HNSCC中的Topo IIalpha基因表达水平高于HNM（P = 0.0298）。此外，HNSCC转移淋巴结样本中的Topo IIalpha mRNA水平显着高于HNM样本（P = 0.0205）。暴露于铂类药物的样本与未暴露于铂类药物的样本之间的六个基因表达水平无显着差异。这些结果表明，使用靶向拓扑异构酶的药物针对HNSCC（尤其是转移性颈部肿瘤）可能有效，并且这些基因在HNSCC中的表达与铂类药物暴露无关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号