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首页> 美国卫生研究院文献>other >Genome-Wide Identification of Mango (Mangifera indica L.) Polygalacturonases: Expression Analysis of Family Members and Total Enzyme Activity During Fruit Ripening
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Genome-Wide Identification of Mango (Mangifera indica L.) Polygalacturonases: Expression Analysis of Family Members and Total Enzyme Activity During Fruit Ripening

机译：芒果（Mangifera indica L.）多半乳糖苷酶的全基因组鉴定：果实成熟过程中家庭成员的表达分析和总酶活性

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摘要

Mango (Mangifera indica L.) is an important commercial fruit that shows a noticeable loss of firmness during ripening. Polygalacturonase (PG, E.C. 3.2.1.15) is a crucial enzyme for cell wall loosening during fruit ripening since it solubilizes pectin and its activity correlates with fruit softening. Mango PGs were mapped to a genome draft using seventeen PGs found in mango transcriptomes and 48 bonafide PGs were identified. The phylogenetic analysis suggests that they are related to Citrus sinensis, which may indicate a recent evolutive divergence and related functions with orthologs in the tree. Gene expression analysis for nine PGs showed differential expression for them during post-harvest fruit ripening, MiPG21-1, MiPG14, MiPG69-1, MiPG17, MiPG49, MiPG23-3, MiPG22-7, and MiPG16 were highly up-regulated. PG enzymatic activity also increased during maturation and these results correlate with the loss of firmness observed in mango during post-harvest ripening, between the ethylene production burst and the climacteric peak. The analysis of PGs promoter regions identified regulatory sequences associated to ripening such as MADS-box, ethylene regulation like ethylene insensitive 3 (EIN3) factors, APETALA2-like and ethylene response element factors. During mango fruit ripening the action of at least these nine PGs contribute to softening, and their expression is regulated at the transcriptional level. The prediction of the tridimensional structure of some PGs showed a conserved parallel beta-helical fold related to polysaccharide hydrolysis and a modular architecture, where exons correspond to structural elements. Further biotechnological approaches could target specific softening-related PGs to extend mango post-harvest shelf life.
机译：芒果（Mangifera indica L.）是一种重要的商业水果，在成熟过程中显示出明显的硬度损失。聚半乳糖醛酸酶（PG，E.C.3.2.1.15）是水果成熟期间细胞壁松弛的关键酶，因为它能溶解果胶，并且其活性与水果软化有关。使用在芒果转录组中发现的17种PG将芒果PG定位到基因组草图，并鉴定了48种纯正PG。系统发育分析表明，它们与柑桔有关，这可能表明最近的进化分歧和与直系同源物在树中的相关功能。对9种PG的基因表达分析显示，在收获后的果实成熟过程中，它们的差异表达，MiPG21-1，MiPG14，MiPG69-1，MiPG17，MiPG49，MiPG23-3，MiPG22-7和MiPG16均被上调。 PG的酶活性在成熟过程中也会增加，这些结果与收获后成熟期间在乙烯产量暴增和更年期高峰之间芒果中硬度的下降有关。 PGs启动子区域的分析确定了与成熟相关的调控序列，如MADS-box，乙烯调控（如乙烯不敏感3（EIN3）因子，APETALA2-like和乙烯响应元件因子）。在芒果果实成熟期间，至少这九种PG的作用有助于软化，并且它们的表达在转录水平受到调控。对某些PG的三维结构的预测表明，与多糖水解有关的保守的平行β-螺旋折叠和模块化结构（外显子对应于结构元素）均如此。进一步的生物技术方法可能针对与软化相关的特定PG，以延长芒果收获后的货架期。

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