• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>other >Selection of housekeeping genes and demonstration of RNAi in cotton leafhopper Amrasca biguttula biguttula (Ishida)
【2h】

Selection of housekeeping genes and demonstration of RNAi in cotton leafhopper Amrasca biguttula biguttula (Ishida)

机译：棉叶蝉Amrasca biguttula biguttula（Ishida）的管家基因的选择和RNAi的演示

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Amrasca biguttula biguttula (Ishida) commonly known as cotton leafhopper is a severe pest of cotton and okra. Not much is known on this insect at molecular level due to lack of genomic and transcriptomic data. To prepare for functional genomic studies in this insect, we evaluated 15 common housekeeping genes (Tub, B-Tub, EF alpha, GADPH, UbiCF, RP13, Ubiq, G3PD, VATPase, Actin, 18s, 28s, TATA, ETF, SOD and Cytolytic actin) during different developmental stages and under starvation stress. We selected early (1st and 2nd), late (3rd and 4th) stage nymphs and adults for identification of stable housekeeping genes using geNorm, NormFinder, BestKeeper and RefFinder software. Based on the different algorithms, RP13 and VATPase are identified as the most suitable reference genes for quantification of gene expression by reverse transcriptase quantitative PCR (RT-qPCR). Based on RefFinder which comprehended the results of three algorithms, RP13 in adults, Tubulin (Tub) in late nymphs, 28S in early nymph and UbiCF under starvation stress were identified as the most stable genes. We also developed methods for feeding double-stranded RNA (dsRNA) incorporated in the diet. Feeding dsRNA targeting Snf7, IAP, AQP1, and VATPase caused 56.17–77.12% knockdown of targeted genes compared to control and 16 to 48% mortality of treated insects when compared to control.
机译：石羊阿姆拉斯卡（Arass bigututula）大叶蝉（Ishida）通常被称为棉叶蝉，是棉花和秋葵的一种严重害虫。由于缺乏基因组和转录组数据，在分子水平上对该昆虫知之甚少。为了准备对该昆虫进行功能基因组研究，我们评估了15种常见的管家基因（Tub，B-Tub，EF alpha，GADPH，UbiCF，RP13，Ubiq，G3PD，VATPase，Actin，18s，28s，TATA，ETF，SOD和细胞溶解性肌动蛋白）在不同的发育阶段和饥饿压力下。我们选择了早期（1 st 和2 nd ），晚期（3 rd 和4 ）阶段的若虫和成人使用geNorm，NormFinder，BestKeeper和RefFinder软件鉴定稳定的管家基因。根据不同的算法， RP13 和 VATPase 被确定为最适合通过逆转录酶定量PCR（RT-qPCR）定量基因表达的参考基因。基于RefFinder理解了成人算法 RP13 ，若虫后期若虫（ Tub ），早期若虫28S和 UbiCF 三种算法的结果在饥饿压力下被确定为最稳定的基因。我们还开发了饲喂饮食中添加的双链RNA（dsRNA）的方法。饲喂靶向Snf7，IAP，AQP1和VATPase的dsRNA，与对照相比，导致靶基因的敲除率为56.17–77.12％，而与对照相比，处理昆虫的死亡率为16％至48％。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号