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The forward and backward transport processes in the AOT/hexane reversed micellar extraction of soybean protein

机译:AOT /己烷逆胶束法提取大豆蛋白中的正向和反向转运过程

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摘要

Soybean protein was taken as a model protein to investigate two aspects of the protein extraction by sodium bis(2-ethylhexyl) sulfosuccinate (AOT) reverse micelles: (1) the forward protein extraction from the solid state, and the effect of pH, AOT concentration, alcohol and water content (W0) on the transfer efficiency; (2) the back-transfer, the capability of the protein to be recovered from the micellar solution. The experimental results led to the conclusion that the highest forward extraction efficiency of soybean protein was reached at AOT concentration 180 mmol l−1, aqueous pH 7.0, KCl concentration 0.05 mol l−1, 0.5 % (v/v) alcohol, W0 18. Under these conditions, the forward extraction efficiency of soybean protein achieved 70.1 %. It was noted that the percentage of protein back extraction depended on the salt concentration and pH value. Around 92 % of protein recovery was obtained after back extraction.
机译:以大豆蛋白为模型蛋白,研究了双(2-乙基己基)磺基琥珀酸钠(AOT)反胶束提取蛋白质的两个方面:(1)从固态中正向提取蛋白质,以及pH,AOT的影响浓度,酒精和水含量(W0)对转移效率的影响; (2)反向转移,即从胶束溶液中回收蛋白质的能力。实验结果表明,在AOT浓度为180 mmol l -1 ,水溶液pH 7.0,KCl浓度为0.05mol l -1时,大豆蛋白的正向提取效率最高。 sup>,0.5%(v / v)醇,W018。在这些条件下,大豆蛋白的正向提取效率达到70.1%。注意到蛋白质反萃取的百分比取决于盐浓度和pH值。反萃取后可回收约92%的蛋白质。

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