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首页> 美国卫生研究院文献>Journal of Food Science and Technology >Culture broth of Ganoderma lucidum exhibited antioxidant antibacterial and α-amylase inhibitory activities
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Culture broth of Ganoderma lucidum exhibited antioxidant antibacterial and α-amylase inhibitory activities

机译：灵芝培养液具有抗氧化抗菌和α-淀粉酶抑制作用

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Culture broth of Ganoderma lucidum was determined for antioxidant, antibacterial and α-amylase inhibitory activities. The culture broth contained protein as determined by Bradford method equaled to 0.2 mg/ml and total phenol content as 0.078 mg GAE/mg protein (0.557 mg GAE/g extract). It exhibited radicals scavenging activities against ABTS+· and DPPH· radicals with a half maximal inhibitory concentration (IC50) less than 1.70 ± 0.02 and 2.28 ± 0.02 µg protein/ml, respectively and reducing power equaled to 4.38 ± 0.02 µmol Trolox/µg protein as investigated by ferric ion reducing antioxidant power method. The culture broth experimented into two approached; (1) treated with pronase and (2) filtered through a membrane with 10 kDa molecular weight cut-off (MWCO). The pronase-treated culture broth exhibited insignificant lower antioxidant activities, but the retained culture broth 10 kDa MWCO resulted in significant decrease in antioxidant activities suggesting that the small proteins might play the key role in antioxidant activity. The culture broth could protect DNA damage from hydroxyl radicals (·OH) generated by Fenton’s reaction. This culture broth showed antibacterial activity towards pathogenic strains Staphylococcus epidermidis and Pseudomonas aeruginosa and also had an interesting α-amylase inhibitory activity. This study suggested that apart from the fruiting bodies and the mycelial of G. lucidum, its culture broth also had potential applications as a value-added ingredient in the product such as in cosmetics and in nutraceuticals.
机译：测定灵芝的培养液的抗氧化，抗菌和α-淀粉酶抑制活性。通过Bradford方法测定，培养液中含有的蛋白质等于0.2 mg / ml，总酚含量为0.078 mg GAE / mg蛋白质（0.557 mg GAE / g提取物）。它显示出对ABTS +·和DPPH ·自由基的清除能力，其半数最大抑制浓度（IC50）小于1.70±0.02和2.28±0.02 µg蛋白/ ml，铁离子还原抗氧化剂功率法研究的还原力分别为4.38±0.02 µmol Trolox / µg蛋白。培养液实验分为两种： （1）用链霉蛋白酶处理，（2）通过截留分子量为10kDa（MWCO）的膜过滤。经链霉蛋白酶处理的培养液显示出较低的抗氧化活性，但保留的培养液10 kDa MWCO导致抗氧化活性显着下降，表明小蛋白可能在抗氧化活性中起关键作用。该培养液可以保护DNA免受Fenton反应产生的羟基自由基（· OH）的破坏。该培养液对表皮葡萄球菌和铜绿假单胞菌具有致病力，并具有令人感兴趣的α-淀粉酶抑制活性。这项研究表明，除了灵芝的子实体和菌丝体，其培养液还可以作为产品中的增值成分，例如在化妆品和营养保健品中潜在应用。

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