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Effects of Dimethylaminoethanol and Compound Amino Acid on D-Galactose Induced Skin Aging Model of Rat

机译:二甲基氨基乙醇和复合氨基酸对D-半乳糖诱导的大鼠皮肤衰老模型的影响

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摘要

A lasting dream of human beings is to reverse or postpone aging. In this study, dimethylaminoethanol (DMAE) and compound amino acid (AA) in Mesotherapy were investigated for their potential antiaging effects on D-galactose induced aging skin. At 18 days after D-gal induction, each rat was treated with intradermal microinjection of saline, AA, 0.1% DMAE, 0.2% DMAE, 0.1% DMAE + AA, or 0.2% DMAE + AA, respectively. At 42 days after treatment, the skin wound was harvested and assayed. Measurement of epidermal and dermal thickness in 0.1% DMAE + AA and 0.2% DMAE + AA groups appeared significantly thicker than aging control rats. No differences were found in tissue water content among groups. Hydroxyproline in 0.1% DMAE + AA, 0.2% DMAE + AA, and sham control groups was much higher than all other groups. Collagen type I, type III, and MMP-1 expression was highly upregulated in both 0.1% DMAE + AA and 0.2% DMAE + AA groups compared with aging control. In contrast, TIMP-1 expression levels of various aging groups were significantly reduced when compared to sham control. Coinjection of DMAE and AA into target tissue has marked antiaging effects on D-galactose induced skin aging model of rat.
机译:人类的长久梦想是逆转或推迟衰老。在这项研究中,研究了Mesotherapy中的二甲基氨基乙醇(DMAE)和复合氨基酸(AA)对D-半乳糖诱导的衰老皮肤的潜在抗衰老作用。 D-gal诱导后第18天,分别对大鼠进行皮内显微注射盐水,AA,0.1%DMAE,0.2%DMAE,0.1%DMAE + AA或0.2%DMAE + AA进行皮内注射。治疗后42天,收获皮肤伤口并进行分析。在0.1%DMAE + AA和0.2%DMAE + AA组中,表皮和真皮厚度的测量值明显比衰老对照组的大鼠厚。各组之间组织水分含量无差异。 0.1%DMAE + AA,0.2%DMAE + AA和假对照组的羟脯氨酸含量均高于所有其他组。与老化对照组相比,在0.1%DMAE + AA和0.2%DMAE + AA组中,I型,III型和MMP-1胶原蛋白的表达均上调。相反,与假对照组相比,各种衰老组的TIMP-1表达水平显着降低。将DMAE和AA共注入目标组织对D-半乳糖诱导的大鼠皮肤衰老模型具有明显的抗衰老作用。

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