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首页> 美国卫生研究院文献>other >Relationship between the Rumen Microbiome and Residual Feed Intake-Efficiency of Brahman Bulls Stocked on Bermudagrass Pastures
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Relationship between the Rumen Microbiome and Residual Feed Intake-Efficiency of Brahman Bulls Stocked on Bermudagrass Pastures

机译：百慕大草牧场放养的婆罗门公牛瘤胃微生物组与剩余饲料摄入效率之间的关系

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Residual feed intake (>RFI) testing has increased selection pressure on biological efficiency in cattle. The objective of this study was to assess the association of the rumen microbiome in inefficient, positive RFI (>p-RFI) and efficient, negative RFI (>n-RFI) Brahman bulls grazing ‘Coastal’ bermudagrass [Cynodondactylon (L.) Pers.]under two levels of forage allowance (high and low stocking intensity). Sixteen Brahman bulls were previously fed in confinement for 70 d to determine the RFI phenotype. Bulls were then allotted 60 d stocking on bermudagrass pastures to estimate RFI using the n-alkane technique. At the conclusion of the grazing period, rumen liquid samples were collected from each bull by stomach tube to evaluate the rumen microbiome. Extraction of DNA, amplification of the V4-V6 region of the 16S rRNA gene, and 454 pyrosequencing were performed on each sample. After denoising the sequences, chimera checking, and quality trimming, 4,573 ± 1,287 sequences were generated per sample. Sequences were then assigned taxonomy from the Greengenes database using the RDP classifier. Overall, 67.5 and 22.9% of sequences were classified as Bacteroidetes and Firmicutes, respectively. Within the phylum Bacteroidetes, Prevotella was the most predominant genus and was observed in greater relative abundance in p-RFI bulls compared with n-RFI bulls (P = 0.01). In contrast, an unidentified Bacteroidales family was greater in relative abundance for n-RFI bulls than p-RFI (26.7 vs. 19.1%; P = 0.03). Ruminococcaceae was the third most abundant family in our samples, but it was not affected by RFI phenotype. No effect of stocking intensity was observed for bacterial taxa, but there was a tendency for alpha diversity and operational taxonomic unit richness to increase with lower stocking intensity. Results suggested the rumen microbiome of p-RFI Brahman bulls has greater levels of Prevotella, but the bacterial community composition was unaffected by stocking intensity.
机译：残留饲料摄入量（> RFI ）测试增加了选择牛对生物效率的压力。这项研究的目的是评估瘤胃微生物组与婆罗门牛低效，正RFI（> p-RFI ）和高效，负RFI（> n-RFI ）的关联。在两个水平的饲草津贴（高和低放养强度）下放牧“沿海”百慕大草（Cynodondactylon（L.）Pers.。先前将16只婆罗门公牛圈养70天，以确定RFI表型。然后，将公牛在百慕大草牧场上放养60天，以使用正构烷烃技术估算RFI。在放牧期结束时，通过胃管从每头公牛收集瘤胃液体样品以评估瘤胃微生物组。对每个样品进行DNA提取，16S rRNA基因的V4-V6区域扩增和454焦磷酸测序。在对序列进行去噪，嵌合体检查和质量修整之后，每个样本生成了4,573±1,287个序列。然后使用RDP分类器从Greengenes数据库为序列分配分类。总体而言，将67.5％和22.9％的序列分别分类为拟杆菌属和Firmicutes。在拟杆菌门内，普雷沃氏菌是最主要的属，与n-RFI公牛相比，p-RFI公牛的相对丰度更高（P = 0.01）。相比之下，一个未鉴定的拟杆菌属在n-RFI公牛的相对丰度要比p-RFI大（26.7比19.1％； P = 0.03）。瘤菌科是我们样品中第三大家族，但不受RFI表型的影响。细菌类群没有观察到放养强度的影响，但是随着放养强度的降低，α多样性和操作分类单位丰富度有增加的趋势。结果表明，p-RFI婆罗门牛的瘤胃微生物组具有较高的Prevotella水平，但细菌群落组成不受放养强度的影响。

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