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Analysis of the Intestinal Lumen Microbiota in an Animal Model of Colorectal Cancer

机译：大肠癌动物模型中肠腔微生物群的分析

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Recent reports have suggested that multiple factors such as host genetics, environment and diet can promote the progression of healthy mucosa towards sporadic colorectal carcinoma. Accumulating evidence has additionally associated intestinal bacteria with disease initiation and progression. In order to examine and analyze the composition of gut microbiota in the absence of confounding influences, we have established an animal model of 1, 2-dimethylhydrazine (DMH)-induced colon cancer. Using this model, we have performed pyrosequencing of the V3 region of the 16S rRNA genes in this study to determine the diversity and breadth of the intestinal microbial species. Our findings indicate that the microbial composition of the intestinal lumen differs significantly between control and tumor groups. The abundance of Firmicutes was elevated whereas the abundance of Bacteroidetes and Spirochetes was reduced in the lumen of CRC rats. Fusobacteria was not detected in any of the healthy rats and there was no significant difference in observed Proteobacteria species when comparing the bacterial communities between our two groups. Interestingly, the abundance of Proteobacteria was higher in CRC rats. At the genus level, Bacteroides exhibited a relatively higher abundance in CRC rats compared to controls (14.92% vs. 9.22%, p<0.001). Meanwhile, Prevotella (55.22% vs. 26.19%), Lactobacillus (3.71% vs. 2.32%) and Treponema (3.04% vs. 2.43%), were found to be significantly more abundant in healthy rats than CRC rats (p<0.001, respectively). We also demonstrate a significant reduction of butyrate-producing bacteria such as Roseburia and Eubacterium in the gut microbiota of CRC rats. Furthermore, a significant increase in Desulfovibrio, Erysipelotrichaceae and Fusobacterium was also observed in the tumor group. A decrease in probiotic species such as Ruminococcus and Lactobacillus was likewise observed in the tumor group. Collectively, we can conclude that a significant difference in intestinal bacterial flora exists between healthy rats and CRC rats.
机译：最近的报告表明，宿主遗传学，环境和饮食等多种因素可以促进健康的粘膜向散发性结直肠癌的发展。越来越多的证据还表明肠道细菌与疾病的发生和发展有关。为了在没有混杂影响的情况下检查和分析肠道菌群的组成，我们建立了由1，2-二甲基肼（DMH）诱导的结肠癌的动物模型。使用该模型，我们在本研究中对16S rRNA基因的V3区进行了焦磷酸测序，以确定肠道微生物的多样性和广度。我们的发现表明，对照组和肿瘤组之间肠道内腔的微生物组成明显不同。在CRC大鼠的管腔中，Firmicutes的丰度升高，而Bacteroidetes和Spirochetes的丰度降低。比较两组之间的细菌群落时，在任何健康的大鼠中均未检测到梭菌，并且观察到的变形杆菌种类也没有显着差异。有趣的是，CRC大鼠中变形杆菌的丰度更高。在属水平上，拟除虫菊酯在CRC大鼠中的丰度比对照组高（14.92％比9.22％，p <0.001）。同时，发现健康大鼠中的Prevotella（55.22％vs. 26.19％），乳酸杆菌（3.71％vs. 2.32％）和梅毒螺旋体（3.04％vs. 2.43％）明显比CRC大鼠丰富（p <0.001，分别）。我们还证明了在CRC大鼠的肠道菌群中，产生丁酸盐的细菌（如Roseburia和Eubacterium）的显着减少。此外，在该肿瘤组中还观察到了脱硫弧菌，大戟科和梭菌的显着增加。在肿瘤组中同样观察到益生菌物种如Ruminococcus和乳杆菌的减少。总的来说，我们可以得出结论，健康大鼠和CRC大鼠之间肠道细菌菌群存在显着差异。

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