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Improved recovery of group A beta-hemolytic streptococci with a new selective medium.

机译:使用新的选择性培养基提高了A组溶血性链球菌的回收率。

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摘要

A new selective group A streptococcus agar (ssA) (BBL Microbiology Systems, Cockeysville, Md.) was evaluated for the recovery and direct plate identification of group A beta-hemolytic streptococci (GABHS) in comparison with sheep blood agar (SBA). A total of 265 GABHS were recovered from 1,116 throat swab specimens on both media, with detection rates of 98.9 and 91.7% for ssA and SBA, respectively. Primary bacitracin disk susceptibility tests were performed on 549 specimens, and presumptive identifications were possible for 81.4% of the GABHS on ssA, as compared with 44.2% on SBA. All of the 120 GABHS recovered from another 567 specimens were identified by a coagglutination method after overnight incubation on ssA, whereas only 70.2% were identified at this interval on SBA. The major advantage of the ssA was the inhibition of normal respiratory flora, permitting improved recovery and rapid identification of GABHS.
机译:与绵羊血琼脂(SBA)相比,评估了一种新的选择性A组链球菌琼脂(ssA)(BBL Microbiology Systems,Cockeysville,MD)的回收率和直接板鉴定A组β-溶血链球菌(GABHS)的能力。在两种培养基上,共从1116个咽拭子样本中回收到265种GABHS,ssA和SBA的检出率分别为98.9和91.7%。在549个标本上进行了杆菌肽敏感性试验,推测可能有ssA的GABHS占81.4%,而SBA占44.2%。在ssA上过夜孵育后,通过凝集法鉴定了从另外567个标本中回收的所有120种GABHS,而在SBA上此时间间隔仅鉴定出70.2%。 ssA的主要优点是抑制了正常的呼吸道菌群,从而提高了恢复能力并能快速鉴定GABHS。

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