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Relative substrate affinity index values: a method for identification of beta-lactamase enzymes and prediction of successful beta-lactam therapy.

机译：相对底物亲和指数值：一种用于鉴定β-内酰胺酶和预测成功的β-内酰胺治疗的方法。

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Using a nitrocefin competition assay, I determined the relative substrate affinity index (RSAI) values of nine clinically significant beta-lactamase enzymes against a range of beta-lactams. Using selected beta-lactam substrates, I observed large differences in the RSAI values of the nine enzymes that were sufficient in many cases to positively identify specific enzymes. I made use of the unique RSAI values of SHV-1, TEM-1, and TEM-2 beta lactamases with cefoxitin to screen for the presence of these enzymes in Klebsiella aerogenes clinical isolates. The RSAI values also allow for the prediction of the outcome of beta-lactam therapy against specific beta-lactamase-producing isolates.

机译：我使用硝菌素竞争测定法，确定了针对一系列β-内酰胺的9种临床上显着的β-内酰胺酶的相对底物亲和力指数（RSAI）值。使用选定的β-内酰胺底物，我观察到这9种酶的RSAI值存在很大差异，这在许多情况下足以肯定地鉴定特定酶。我利用了带有头孢西丁的SHV-1，TEM-1和TEM-2β内酰胺酶的独特RSAI值来筛选产气克雷伯氏菌临床分离物中这些酶的存在。 RSAI值还可以预测针对产生特定β-内酰胺酶的分离物的β-内酰胺治疗的结果。

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期刊名称 Journal of Clinical Microbiology
作者
R James;
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作者单位

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年(卷),期 1983(17),5
年度 1983
页码 791–798
总页数 8
原文格式 PDF
正文语种
中图分类微生物学;
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专利

1. Relative substrate affinity index values: a method for identification of beta-lactamase enzymes and prediction of successful beta-lactam therapy. [J] . R James Journal of Clinical Microbiology . 1983,第5期

机译：相对底物亲和指数值：鉴定β-内酰胺酶和预测成功的β-内酰胺治疗的方法。
2. Loss of enzyme activity during turnover of the Bacillus cereus beta-lactamase catalysed hydrolysis of beta-lactams due to loss of zinc ion [J] . Badarau A, Page MI Journal of biological inorganic chemistry: JBIC: a publication of the Society of Biological Inorganic Chemistry . 2008,第6期

机译：蜡状芽孢杆菌β-内酰胺酶催化的水解过程中酶活性的损失是由于锌离子的损失
3. Identification of tubulin drug binding sites and prediction of relative differences in binding affinities to tubulin isotypes using digital signal processing [J] . Chen K, Huzil JT, Freedman H, Journal of molecular graphics & modelling . 2008,第4期

机译：使用数字信号处理鉴定微管蛋白药物结合位点并预测与微管蛋白同种型的结合亲和力的相对差异
4. SUCCESSFUL EXAMPLES OF THE APPLICATION OF NOVEL ITERATIVE TRAINABLE ALGORITHMS TO GUIDE RATIONAL MUTATION STRATEGIES FOR ENZYME ENGINEERING: FROM PREDICTION TO LAB TESTING TO ALGORITHM RETRAINING [C] . Alvaro Olivera-Nappa, Barbara A. Andrews, Juan A. Asenjo Enzyme Engineering Conference . 2018

机译：新型迭代培训算法应用的成功示例，以指导酶工程的理性突变策略：从预测到实验室测试到算法再训练
5. D-alanine:D-alanine ligase, cephalosporin beta-lactamase, and complexes of new beta-lactams with their target enzyme: X-ray structural studies. [D] . Liu, Hansong. 1990

机译：D-丙氨酸：D-丙氨酸连接酶，头孢菌素β-内酰胺酶，以及新型β-内酰胺与目标酶的复合物：X射线结构研究。
6. Iodometric Assay Method for Beta-Lactamase with Various Beta-Lactam Antibiotics as Substrates [O] . Tetsuo Sawai, Ikuko Takahashi, Saburo Yamagishi 1978

机译：以各种β-内酰胺类抗生素为底物的β-内酰胺酶的碘量法
7. Relative substrate affinity index values: a method for identification of beta-lactamase enzymes and prediction of successful beta-lactam therapy [O] . R James 1983

机译：相对底物亲和力指标值：一种鉴定β-内酰胺酶的方法和成功β-内酰胺疗法的预测
8. Botulinum Neurotoxin Serotype F: Identification of Substrate Recognition Requirements and Development of Inhibitors with Low Nanomolar Affinity [R] . Schmidt, J. J. , Stafford, R. G. 2005

机译：肉毒杆菌神经毒素血清型F：基质识别要求的鉴定和具有低纳摩尔亲和力的抑制剂的开发

Relative substrate affinity index values: a method for identification of beta-lactamase enzymes and prediction of successful beta-lactam therapy.

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