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Long term hypoxia during gestation alters perirenal adipose tissue gene expression in the lamb

机译：妊娠期长期缺氧改变羔羊肾周围脂肪组织基因表达

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We previously reported that following long-term hypoxia (LTH), the ovine foetus exhibits enhanced expression of brown/beige adipose genes. This study was designed to determine if these changes are preserved after birth. Pregnant ewes were divided among three groups, 1) Control, sea level, 2) LTH, high altitude (3,820 m, LTH-HA) from ~ day 40 of gestation through ~14 days post-delivery and 3) LTH from ⁓ day 40 through day 137 of gestation then returned to the laboratory where atory reduced maternal PO was maintained by nitrogen infusion. Following delivery, lambs remained at sea level (LTH-SL). Perirenal adipose tissue was collected at ~day 14, and qRT-PCR was used to quantify mRNA. Uncoupling protein 1 (UCP-1), PPAR gamma coactivator 1 (PGC1α), and deiodinase-2 (DIO2) mRNA levels were significantly lower in both LTH groups while PR domain containing 16 (PRDM16) levels did not differ. Peroxisome proliferator-activated receptor (PPARγ) was maintained in the LTH-HA group and significantly increased in the LTH-SL group, compared to control. Unlike our previous LTH foetal studies, the brown/beige fat phenotype was rapidly lost by day 14 postpartum compared to control, while PPARγ was maintained. This loss of the brown fat phenotype may promote obesity due to decreased energy expenditure, favouring fat deposition.
机译：我们以前曾报道过长期缺氧（LTH）后，绵羊胎儿的棕色/米色脂肪基因表达增强。本研究旨在确定这些变化在出生后是否得以保留。将怀孕的母羊分为三组：1）对照，海平面，2）LTH，从妊娠〜40天到分娩后〜14天的高海拔地区（3,820 m，LTH-HA）和3）从⁓40天起的LTH到妊娠第137天，然后回到实验室，通过输注氮来维持母体摄食量降低。交付后，羔羊仍处于海平面（LTH-SL）。在约第14天收集肾周脂肪组织，并使用qRT-PCR定量mRNA。在两个LTH组中，解偶联蛋白1（UCP-1），PPARγ共激活因子1（PGC1α）和脱碘酶2（DIO2）mRNA的水平均显着降低，而PR结构域中的16（PRDM16）水平没有差异。与对照组相比，LTH-HA组维持了过氧化物酶体增殖物激活受体（PPARγ），LTH-SL组显着增加。与我们之前的LTH胎儿研究不同，与对照组相比，产后第14天棕色/米色脂肪表型迅速消失，而PPARγ保持不变。褐色脂肪表型的这种丧失可能由于减少的能量消耗而促进肥胖，有利于脂肪沉积。

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