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首页> 外文期刊>Progress in Artificial Intelligence >Long term hypoxia during gestation alters perirenal adipose tissue gene expression in the lamb
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Long term hypoxia during gestation alters perirenal adipose tissue gene expression in the lamb

机译：在妊娠期间长期缺氧改变肝癌脂肪组织基因在羊羔中的表达

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摘要

We previously reported that following long-term hypoxia (LTH), the ovine foetus exhibits enhanced expression of brown/beige adipose genes. This study was designed to determine if these changes are preserved after birth. Pregnant ewes were divided among three groups, 1) Control, sea level, 2) LTH, high altitude (3,820 m, LTH-HA) from day 40 of gestation through 14 days post-delivery and 3) LTH from & x2053; day 40 through day 137 of gestation then returned to the laboratory where atory reduced maternal PO2 was maintained by nitrogen infusion. Following delivery, lambs remained at sea level (LTH-SL). Perirenal adipose tissue was collected at day 14, and qRT-PCR was used to quantify mRNA. Uncoupling protein 1 (UCP-1), PPAR gamma coactivator 1 (PGC1 alpha), and deiodinase-2 (DIO2) mRNA levels were significantly lower in both LTH groups while PR domain containing 16 (PRDM16) levels did not differ. Peroxisome proliferator-activated receptor (PPAR gamma) was maintained in the LTH-HA group and significantly increased in the LTH-SL group, compared to control. Unlike our previous LTH foetal studies, the brown/beige fat phenotype was rapidly lost by day 14 postpartum compared to control, while PPAR gamma was maintained. This loss of the brown fat phenotype may promote obesity due to decreased energy expenditure, favouring fat deposition.
机译：我们之前报道，在长期缺氧（LTH）之后，绵羊胎儿表现出增强的棕色/米色脂肪基因的表达。本研究旨在确定出生后是否保留了这些变化。怀孕的母羊分为三组，1）控制，海平面，2）Lth，高海拔（3,820米，Lth-HA）从妊娠第40天通过递送后的14天和3）来自＆x2053;在妊娠的第40天至第137天，然后返回到实验室，其中通过氮输注维持物质的母体PO2。交货后，羔羊仍处于海平面（Lth-S1）。在第14天收集胆汁脂肪组织，并使用QRT-PCR来定量mRNA。在第一个蛋白1（UCP-1）中，PPARγγ共粘膜1（PGC1α）和Dei联酶-2（DIO2）mRNA水平在含有16（PRDM16）水域的PR结构域并没有区别。与对照相比，在Lth-HA组中维持过氧化物体增殖剂活化受体（PPARγ）并在Lth-SL组中显着增加。与我们之前的第一个胎儿研究不同，与对照相比，棕色/米色脂肪表型迅速损失，而PPARγ的维持。由于能量消耗降低，脂肪沉积，这种棕色脂肪表型的这种损失可能会促进肥胖。

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