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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Characterization of Monkey Enteropathogenic Escherichia coli (EPEC) and Human Typical and Atypical EPEC Serotype Isolates from Neotropical Nonhuman Primates
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Characterization of Monkey Enteropathogenic Escherichia coli (EPEC) and Human Typical and Atypical EPEC Serotype Isolates from Neotropical Nonhuman Primates

机译：猴肠道致病性大肠杆菌（EPEC）和新热带非人类灵长类动物的人类典型和非典型EPEC血清型分离株的表征

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Enteropathogenic Escherichia coli (EPEC) has been associated with infantile diarrhea and mortality in humans in developing countries. While diarrhea is also a major problem among primates kept in captivity, the role of E. coli is unclear. This study was designed to characterize diarrheagenic E. coli recovered from the feces of 56 New World nonhuman primates, primarily marmosets (Callithrix spp.). Seventeen of the 56 primates had signs of diarrhea and/or enteritis. E. coli recovered from feces from these animals was tested by PCR for genes encoding virulence factors of diarrheagenic E. coli and for patterns of adherence to HeLa cells. In addition, isolates were characterized by the fluorescence actin staining test and by their ability to induce attaching and effacing lesions. PCR for the eae gene was positive in 10 of the 39 (27%) apparently healthy animals and in 8 of the 17 (47%) animals with diarrhea and/or enteritis. Colonies of eae+ E. coli were serotyped and examined by PCR for genes encoding EPEC virulence markers. The eae+ E. coli isolates recovered from both healthy and sick nonhuman primates demonstrated virulence-associated attributes similar to those of EPEC strains implicated in human disease and are designated monkey EPEC. The results presented here indicate that EPEC may be a significant pathogen for nonhuman primates, deserving further investigation. The similarities between the affected animals investigated in this study and human EPEC infections suggest that marmosets may represent an important model for EPEC in humans.
机译：肠道致病性大肠杆菌（EPEC）与发展中国家的婴儿腹泻和人类死亡率相关。腹泻也是人工饲养的灵长类动物的主要问题，但大肠杆菌的作用尚不清楚。这项研究旨在表征从56个新世界非人类灵长类动物（主要是mar猴）的粪便中回收的腹泻性大肠杆菌。 56个灵长类动物中有17个有腹泻和/或肠炎的迹象。通过PCR测试从这些动物的粪便中回收的大肠杆菌，以检测编码腹泻性大肠杆菌的致病因子的基因以及对HeLa细胞的粘附模式。此外，分离物的特征还在于荧光肌动蛋白染色试验及其诱导附着和脱落损伤的能力。在39个明显健康的动物中，有10个（27％）和在腹泻和/或肠炎的17个动物中，有8个（47％）的eae基因PCR阳性。对eae + 大肠杆菌菌落进行血清分型，并通过PCR检查编码EPEC毒力标记的基因。从健康和生病的非人类灵长类动物身上回收的eae + 大肠杆菌分离株显示出与毒力相关的特性，与涉及人类疾病的EPEC菌株相似，被命名为猴子EPEC。这里给出的结果表明，EPEC可能是非人类灵长类动物的重要病原体，值得进一步研究。在这项研究中研究的患病动物与人类EPEC感染之间的相似性表明，mar猴可能代表了人类EPEC的重要模型。

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