Rapid Identification and Differentiation of Clinical Isolates of Enteropathogenic Escherichia coli (EPEC), Atypical EPEC, and Shiga Toxin-Producing Escherichia coli by a One-Step Multiplex PCR Method

Daniel Müller; Peter Hagedorn; Sabine Brast; Gerhard Heusipp; Martina Bielaszewska; Alexander W. Friedrich; Helge Karch; M. Alexander Schmidt

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Rapid Identification and Differentiation of Clinical Isolates of Enteropathogenic Escherichia coli (EPEC), Atypical EPEC, and Shiga Toxin-Producing Escherichia coli by a One-Step Multiplex PCR Method

机译：一步多重PCR方法快速鉴定和区分肠致病性大肠杆菌（EPEC），非典型EPEC和产志贺毒素的大肠杆菌

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Enteropathogenic Escherichia coli (EPEC), atypical enteropathogenic E. coli, and Shiga toxin-producing E. coli differ in their virulence factor profiles, clinical manifestations, and prognosis, and they require different therapeutic measures. We developed and evaluated a robust multiplex PCR to identify these pathogroups based on sequences complementary to escV, bfpB, stx₁, and stx₂.

机译：肠致病性大肠埃希菌（EPEC），非典型性肠致病性大肠埃希菌。大肠杆菌和产生志贺毒素的 E。大肠杆菌的毒力因子概况，临床表现和预后不同，因此需要不同的治疗措施。我们开发并评估了鲁棒的多重PCR，以基于与 escV ， bfpB ， stx _{1 和 stx _{2 。}} 展开▼

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《Journal of Clinical Microbiology》 |2006年第7期|共4页

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Daniel Müller; Peter Hagedorn; Sabine Brast; Gerhard Heusipp; Martina Bielaszewska; Alexander W. Friedrich; Helge Karch; M. Alexander Schmidt;
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中图分类医学微生物学（病原细菌学、病原微生物学）;

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1. Rapid Identification and Differentiation of Clinical Isolates of Enteropathogenic Escherichia coli (EPEC), Atypical EPEC, and Shiga Toxin-Producing Escherichia coli by a One-Step Multiplex PCR Method [J] . Daniel Müller, Peter Hagedorn, Sabine Brast, Journal of Clinical Microbiology . 2006,第7期

机译：一步多重PCR方法快速鉴定和区分肠致病性大肠杆菌（EPEC），非典型EPEC和产志贺毒素的大肠杆菌

2. Genetic characterization of Shiga toxin-producing Escherichia coli (STEC) and atypical enteropathogenic Escherichia coli (EPEC) isolates from goat's milk and goat farm environment [J] . Alvarez-Suarez Maria-Elena, Otero Andres, Garcia-Lopez Maria-Luisa, International Journal of Food Microbiology . 2016,第Null期

机译：产自山羊奶和山羊养殖场的滋贺毒素生产性大肠杆菌（STEC）和非典型肠致病性大肠杆菌（EPEC）分离株的遗传特征

3. Subtype analysis of stx1, stx2 and eae genes in Shiga toxin-producing Escherichia coli (STEC) and typical and atypical enteropathogenic E. coli (EPEC) from lambs in India [J] . Wani S.A., Hussain I., Fayaz I., The Veterinary Journal . 2009,第3期

机译：印度产羔羊毒素的大肠杆菌（STEC）和典型和非典型肠致病性大肠杆菌（EPEC）中stx1，stx2和eae基因的亚型分析

4. Metaproteomics Analyses of the Secretome of Enterohemorrhagic and Enteropathogenic Escherichia Coli (EHEC And EPEC) Strains in Biological Matrices [C] . Rabih E. Jabbour, Vicky H Bevilacqua, Samir V. Deshpande, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2012

机译：生物学基质中肠溶性和肠球脑肠外大肠杆菌（EHEC和EPEC）菌株的exercaproomics分析

5. Understanding the Role of Enteropathogenic Escherichia coli (EPEC) in Immunosuppressed Cancer Patients [D] . Olvera, Adilene 2018

机译：了解肠病原性大肠杆菌（EPEC）在免疫抑制的癌症患者中的作用

6. Rapid Identification and Differentiation of Clinical Isolates of Enteropathogenic Escherichia coli (EPEC) Atypical EPEC and Shiga Toxin-Producing Escherichia coli by a One-Step Multiplex PCR Method [O] . Daniel Müller, Peter Hagedorn, Sabine Brast, 2006

机译：一步多重PCR方法快速鉴定和区分肠致病性大肠杆菌（EPEC）非典型EPEC和产志贺毒素的大肠杆菌

7. Rapid Identification and Differentiation of Clinical Isolates of Enteropathogenic Escherichia coli (EPEC), Atypical EPEC, and Shiga Toxin-Producing Escherichia coli by a One-Step Multiplex PCR Method [O] . Müller, Daniel, Hagedorn, Peter, Brast, Sabine, 2006

机译：一步多重PCR方法快速鉴定和区分肠致病性大肠杆菌（EPEC），非典型EPEC和产志贺毒素的大肠杆菌

1. 多重PCR方法检测鸭源产志贺氏毒素大肠杆菌 [J] . 王英 ,汤承 ,于学辉 . 中国预防兽医学报 . 2009,第010期

2. 肠致病性大肠杆菌(EPEC)的致病机理 [J] . 宦海霞 ,高崧 ,刘秀梵 . 中国人兽共患病学报 . 2006,第006期

3. 多重PCR方法械检测EHEC和EPEC毒力基因 [J] . 潘劲草 ,黄志成 ,孟冬梅 . 中国人兽共患病学报 . 2001,第001期

4. 检测产志贺毒素大肠杆菌的菌落PCR方法 [J] . 李睿 ,许芳 ,张忠美 . 食品科学 . 2010,第016期

5. 肠道致病性大肠埃希氏菌（EPEC）和产肠毒素大肠... [J] . 邓涤夷 ,林成水 . 中国人兽共患病杂志 . 1992,第005期

6. 检测产志贺毒素大肠杆菌的四重PCR方法的建立 [C] . . 中国畜牧兽医学会兽医公共卫生学分会成立大会暨第一次学术研讨会 . 2008

7. 产志贺毒素多重耐药大肠杆菌噬菌体的分离、生物学特性及全基因组分析 [A] . 韦德源 . 2020

1. 新型产志贺毒素F18型大肠杆菌噬菌体Esc-COP-1及其用于抑制产志贺毒素F18型大肠杆菌增殖的用途 [P] . 中国专利： CN107208068B . 2021.04.20

2. 新型产志贺毒素F18型大肠杆菌噬菌体Esc‑COP‑1及其用于抑制产志贺毒素F18型大肠杆菌增殖的用途 [P] . 中国专利： CN107208068A . 2017-09-26

3. Enteropathogenic Escherichia coli EPEC Enteroinvasive Escherichia coli EIEC screening primer PNA probe Real-time multiplex PCR Screening method of Enteropathogenic Escherichia coli and Enteroinvasive Escherichia coli associated gastrointestinal infection by PNA based real-time PCR [P] . 外国专利： KR102027157B1 . 2019-10-01

机译：肠致病性大肠埃希氏菌EPC

4. Development of a Singleplex Real-Time PCR Kit for Rapid Detection of Enteropathogenic Escherichia coli (EPEC) jkp, bfpA target gene [P] . 外国专利： KR1020200059763A . 2020.05.29

5. DEVELOPMENT OF SINGLEPLEX REAL-TIME PCR KIT CAPABLE OF QUICKLY DETECTING ENTEROPATHOGENIC ESCHERICHIA COLI (EPEC) USING TARGET GENE [P] . 外国专利： KR20200059763A . 2020-05-29

机译：靶向基因快速检测肠病性大肠杆菌的单实时PCR试剂盒的开发

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Rapid Identification and Differentiation of Clinical Isolates of Enteropathogenic Escherichia coli (EPEC), Atypical EPEC, and Shiga Toxin-Producing Escherichia coli by a One-Step Multiplex PCR Method

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