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首页> 外文期刊>The Veterinary Journal >Subtype analysis of stx1, stx2 and eae genes in Shiga toxin-producing Escherichia coli (STEC) and typical and atypical enteropathogenic E. coli (EPEC) from lambs in India
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Subtype analysis of stx1, stx2 and eae genes in Shiga toxin-producing Escherichia coli (STEC) and typical and atypical enteropathogenic E. coli (EPEC) from lambs in India

机译:印度产羔羊毒素的大肠杆菌(STEC)和典型和非典型肠致病性大肠杆菌(EPEC)中stx1,stx2和eae基因的亚型分析

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摘要

Seventy-five Escherichia coli isolates with at least one targeted virulence gene were recovered from 338 lambs with (n = 230) and without (n = 108) diarrhoea. The isolates belonged to 36 different serogroups. Shiga toxin-producing E. coli (STEC) was isolated from 9.6% of lambs with and 24.1% of lambs without diarrhoea. Enteropathogenic E. coli (EPEC) was isolated from 6.1% of lambs with and 11.1% of lambs without diarrhoea. Of 26 EPEC isolates, seven were typical (positive for bfpA), and, of 34 stx1 positive isolates, 25 were subtyped as stx1c. Five of 29 stx2 positive isolates were subtyped as stx2d and two as stx2c. Seven of 45 eae positive isolates were subtyped as eae subtype zeta (eaee). This appears to be the first report of the isolation of typical EPEC from sheep in India.
机译:从338只有(n = 230)和没有(n = 108)腹泻的羔羊中回收了75个带有至少一个靶向毒力基因的大肠杆菌分离株。分离物属于36个不同的血清群。从9.6%有腹泻的羔羊和24.1%无腹泻的羔羊中分离出产生志贺毒素的大肠杆菌(STEC)。从6.1%有腹泻的羔羊和11.1%无腹泻的羔羊中分离出肠道致病性大肠杆菌(EPEC)。在26个EPEC分离株中,有7个是典型的(对bfpA呈阳性),在34个stx1阳性分离株中,有25个亚型为stx1c。 29个stx2阳性分离株中有五个亚型为stx2d,两个为stx2c。 45个eae阳性分离株中有7个亚型为eae亚型zeta(eaee)。这似乎是印度从绵羊中分离出典型的EPEC的首次报道。

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