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首页> 美国卫生研究院文献>International Journal of Environmental Research and Public Health >Removal of Pollutants in Mine Wastewater by a Non-Cytotoxic Polymeric Bioflocculant from Alcaligenes faecalis HCB2
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Removal of Pollutants in Mine Wastewater by a Non-Cytotoxic Polymeric Bioflocculant from Alcaligenes faecalis HCB2

机译：粪粪产碱菌HCB2的非细胞毒性高分子生物絮凝剂去除矿山废水中的污染物

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Bioflocculation is a physicochemical technique often employed to efficiently remove colloidal water pollutants. Consequently, in this study, a bioflocculant was produced, characterised and applied to remove pollutants in mine wastewater. The maximum flocculation activity of 92% was recorded at 30 °C, pH 9.0 when maltose and urea were used as energy sources and 72 h of fermentation at the inoculum size of 1% ( / ). K proved to be a favourable cation. The bioflocculant yield of 4 g/L was obtained. Scanning electron microscopy illustrated a hexagonal-like structure of the bioflocculant. It is composed of carbohydrates and proteins in mass proportion of 88.6 and 9.5%, respectively. The Fourier transform infrared spectrum revealed the presence of hydroxyl, amide and amino functional groups. More than 73% of the bioflocculant was obtained after exposure to 600 °C using the thermogravimetric analyser. Human embryonic kidney 293 (HEK 293) cells exhibited 95% viability after being treated with 200 µg/µL of the bioflocculant. The flocculation mechanisms were proposed to be as a result of a double layer compression by K , chemical reactions and bridging mechanism. The removal efficiencies of 59, 72, and 75% on biological oxygen demand, chemical oxygen demand and sulphur, were obtained respectively. Thus, the bioflocculant have potential use in wastewater treatment.
机译：生物絮凝是一种经常用于有效去除胶体水污染物的物理化学技术。因此，在这项研究中，生产了一种生物絮凝剂，进行了表征并应用于去除矿山废水中的污染物。当以麦芽糖和尿素为能源，在接种量为1％（/）的条件下发酵72小时时，在30°C，pH 9.0时记录到最大絮凝活性为92％。 K被证明是一个有利的阳离子。获得的生物絮凝剂产量为4 g / L。扫描电子显微镜显示了生物絮凝剂的六边形结构。它由碳水化合物和蛋白质组成，质量比例分别为88.6和9.5％。傅立叶变换红外光谱揭示了羟基，酰胺和氨基官能团的存在。使用热重分析仪暴露于600°C后，获得了超过73％的生物絮凝剂。用200 µg / µL的生物絮凝剂处理后，人类胚胎肾293（HEK 293）细胞表现出95％的活力。提出的絮凝机理是由于K双层压缩，化学反应和桥连机理的结果。对生物需氧量，化学需氧量和硫的去除效率分别为59、72和75％。因此，该生物絮凝剂在废水处理中具有潜在用途。

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