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Characterization of a Novel Melt Curve by Use of the Roche LightCycler HSV 1/2 Analyte-Specific Reagent Real-Time PCR Assay: Frequencies of This Novel (Low) Melt Curve and Commonly Encountered (Intermediate) Melt Curves

机译：使用Roche LightCycler HSV 1/2分析物特异性试剂实时PCR分析法表征新型熔解曲线：此新型（低）熔解曲线和常见（中级）熔解曲线的频率

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摘要

We characterize a novel probe binding-site polymorphism detectable solely by melt curve analysis using the Roche LightCycler HSV 1/2 analyte-specific reagent real-time PCR assay. The frequencies of this novel (47°C) and previously described intermediate (60 to 62°C) melt curves were 0.016% and 4.9%, respectively.

机译：我们表征了仅通过使用Roche LightCycler HSV 1/2分析物特异性试剂实时PCR测定法的熔解曲线分析就可以检测到的新型探针结合位点多态性。该新颖的（47℃）和先前描述的中间温度（60至62℃）的熔解曲线的频率分别为0.016％和4.9％。

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期刊名称 Journal of Clinical Microbiology
作者
Bobby L. Boyanton Jr.; Amro Almradi; Mark J. Espy; Anne E. Prada; John P. Gibson; Bobbi S. Pritt;
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作者单位

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年(卷),期 2014(52),3
年度 2014
页码 957–959
总页数 3
原文格式 PDF
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中图分类微生物学;
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1. Characterization of a Novel Melt Curve by Use of the Roche LightCycler HSV 1/2 Analyte-Specific Reagent Real-Time PCR Assay: Frequencies of This Novel (Low) Melt Curve and Commonly Encountered (Intermediate) Melt Curves [J] . Bobby L. Boyanton, Amro Almradi, Mark J. Espy, Journal of Clinical Microbiology . 2014,第3期

机译：使用Roche LightCycler HSV 1/2分析物特异性试剂实时PCR分析法表征新型熔解曲线：该新型（低）熔解曲线和常见的（中等）熔解曲线的频率
2. HPV Genotyping of Solid Tumors Using Real-Time PCR and Multi-Color Melt Curve AnalysisHPV Genotyping of Solid Tumors Using Real-Time PCR and Multi-Color Melt Curve Analysis [J] . Atkinson A., Studwell C. M., Tafe L. J., The Journal of molecular diagnostics: JMD . 2017,第6期

机译：使用实时PCR的实时PCR和多色熔体曲线分析实时PCR和多色熔体曲线分析的HPV基因分型
3. Multiplex high resolution melt-curve real-time PCR assay for reliable detection of Salmonella [J] . Food Control . 2018,第期

机译：多重高分辨率熔融曲线实时PCR测定，可靠地检测沙门氏菌
4. Prediction of RTD Curves for Non-isotherm Steel Melt Flows in Continuous Casting Tundish [C] . M. Alizadeh, H. Edris International Symposium on Liquid Metal Processing and Casting . 2007

机译：连续铸造中间包中的非等温钢熔体流动RTD曲线的预测
5. A real-time ARMS PCR/high-resolution melt curve assay for the detection of the three primary mitochondrial mutations in Leber’s hereditary optic neuropathy [O] . Siobhan Eustace Ryan, Fergus Ryan, Veronica O’Dwyer, 2016

机译：实时ARMS PCR /高分辨率熔解曲线测定法可检测Leber遗传性视神经病变中的三个主要线粒体突变
6. PL-006 Study on detection of resistant genes in common clinical bacteria used a multiplex real-time PCR and melt temperature curve assay [O] . Lu Xuedong, Xia Chengjing, Huang Lie, 2009

机译：PL-006多重实时荧光定量PCR和熔解温度曲线测定法检测常见临床细菌耐药基因的研究

Characterization of a Novel Melt Curve by Use of the Roche LightCycler HSV 1/2 Analyte-Specific Reagent Real-Time PCR Assay: Frequencies of This Novel (Low) Melt Curve and Commonly Encountered (Intermediate) Melt Curves

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