• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Polymers >PEGylated Thermo-Sensitive Bionic Magnetic Core-Shell Structure Molecularly Imprinted Polymers Based on Halloysite Nanotubes for Specific Adsorption and Separation of Bovine Serum Albumin
【2h】

PEGylated Thermo-Sensitive Bionic Magnetic Core-Shell Structure Molecularly Imprinted Polymers Based on Halloysite Nanotubes for Specific Adsorption and Separation of Bovine Serum Albumin

机译：基于埃洛石纳米管的聚乙二醇化热敏仿生磁核-壳结构分子印迹聚合物对牛血清白蛋白的特异性吸附和分离

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Novel PEGylated thermo-sensitive bionic magnetic core-shell structure molecularly imprinted polymers (PMMIPs) for the specific adsorption and separation of bovine serum albumin (BSA) were obtained via a surface-imprinting technique. X-ray diffraction (XRD), scanning electron microscopy (SEM), transmission electron microscopy (TEM), vibrating sample magnetometry (VSM), fourier transform infrared spectrometry (FT-IR), thermal gravimetric analysis (TGA), and specific surface area (BET), were adopted to demonstrate that novel PMMIPs were successfully synthesized. Subsequently, the prepared PMMIPs were used as the extractor for BSA and were combined with magnetic solid-phase extraction. The concentrations of BSA were detected by UV-vis spectrophotometry at 278 nm. The maximum adsorption capacity of the PMMIPs was 258 mg g , which is much higher than that of non-imprinted polymer (PMNIPs). PMMIPs showed favorable selectivity for BSA against reference proteins, i.e., bovine hemoglobin, ovalbumin and lysozyme. PMMIPs were further used to recognize BSA in protein mixtures, milk, urine and sewage, these results revealed that approximately 96% of the ideal-state adsorption capacity of PMMIPs for BSA was achieved under complicated conditions. Regeneration and reusability studies demonstrated that adsorption capacity loss of the PMMIPs was not obvious after recycling for four times. Facile synthesis, excellent adsorption property and efficient selectivity for BSA trapping are features that highlight PMMIPs as an attractive candidate for biomacromolecular purification.
机译：通过表面印迹技术获得了用于牛血清白蛋白（BSA）特异性吸附和分离的新型聚乙二醇化热敏仿生磁核壳结构分子印迹聚合物（PMMIPs）。 X射线衍射（XRD），扫描电子显微镜（SEM），透射电子显微镜（TEM），振动样品磁强度（VSM），傅立叶变换红外光谱（FT-IR），热重分析（TGA）和比表面积（BET），被用来证明新型PMMIPs已成功合成。随后，将制备的PMMIPs用作BSA的提取器，并与磁性固相提取法结合。通过UV-可见分光光度法在278nm处检测BSA的浓度。 PMMIPs的最大吸附容量为258 mg g，远高于非印迹聚合物（PMNIPs）的最大吸附容量。 PMMIP对BSA对参考蛋白，即牛血红蛋白，卵清蛋白和溶菌酶显示出良好的选择性。 PMMIPs还可用于识别蛋白质混合物，牛奶，尿液和污水中的BSA，这些结果表明，在复杂条件下，PMMIPs对BSA的理想状态吸附能力约为96％。再生和可重复使用性研究表明，PMMIPs吸附四次后，其吸附容量损失并不明显。简便的合成，出色的吸附性能和对BSA捕集的高效选择性是突出PMMIPs作为生物大分子纯化有吸引力的候选物的特征。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号