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首页> 美国卫生研究院文献>Plants >Eukaryotic and Prokaryotic Phytochelatin Synthases Differ Less in Functional Terms Than Previously Thought: A Comparative Analysis of Marchantia polymorpha and Geitlerinema sp. PCC 7407
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Eukaryotic and Prokaryotic Phytochelatin Synthases Differ Less in Functional Terms Than Previously Thought: A Comparative Analysis of Marchantia polymorpha and Geitlerinema sp. PCC 7407

机译：真核和原核植物精致酶的功能性术语少于先前思考的功能较少：Marchantia多晶型和Geitlerinema Sp的比较分析。 PCC 7407.

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摘要

This paper reports functional studies on the enzyme phytochelatin synthase in the liverwort and the cyanobacterium sp. strain PCC 7407. In vitro activity assays in control samples (cadmium-untreated) showed that phytochelatin synthase was constitutively expressed in both organisms. In the presence of 100 µM cadmium, in both the liverwort and the cyanobacterium, the enzyme was promptly activated in vitro, and produced phytochelatins up to the oligomer PC . Likewise, exposure to 10–36 µM cadmium for 6-120 h induced in both organisms phytochelatin synthesis up to PC . Furthermore, the glutathione (GSH) levels in were constitutively low (compared with the average content in higher plants), but increased considerably under cadmium stress. Conversely, the GSH levels in sp. PCC 7407 were constitutively high, but were halved under metal treatments. At odds with former papers, our results demonstrate that, as in and other plants, the cyanobacterial phytochelatin synthase exposed to cadmium possesses manifest transpeptidasic activity, being able to synthesize phytochelatins with a degree of oligomerization higher than PC . Therefore, prokaryotic and eukaryotic phytochelatin synthases differ less in functional terms than previously thought.
机译：本文报告了肝曲线和蓝藻酶中酶植物精素合成酶的功能研究。菌株PCC 7407.对照样品中的体外活性测定（镉 - 未处理）显示，在两种生物体中植物切割素合成酶组成型。在100μm镉的存在下，在肝曲线和蓝藻中，酶在体外迅速激活，并将植物切氨基盐产生给低聚物PC。同样地，暴露于10-36μm的镉，在两种生物中诱导的6-120小时植物植物精致素合成到PC。此外，谷胱甘肽（GSH）水平的组成型低（与高等植物的平均含量相比），但在镉胁迫下显着增加。相反，SP的GSH水平。 PCC 7407组成型高，但在金属处理下减半。与前论文的赔率有所不同，我们的结果表明，如在和其他植物中，暴露于镉的蓝细菌植物精素合成酶具有表现出的型传递型活性，能够合成具有高于PC的寡聚化程度的植物植物素。因此，原核和真核植物素合酶的功能术语差异比以前思想的不同。

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