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Deciphering the Novel Role of AtMIN7 in Cuticle Formation and Defense against the Bacterial Pathogen Infection

机译:解析ATMIN7在皮层形成和防御对细菌病原体感染的新作用

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摘要

The cuticle is the outermost layer of plant aerial tissue that interacts with the environment and protects plants against water loss and various biotic and abiotic stresses. ADP ribosylation factor guanine nucleotide exchange factor proteins (ARF-GEFs) are key components of the vesicle trafficking system. Our study discovers that AtMIN7, an ARF-GEF, is critical for cuticle formation and related leaf surface defense against the bacterial pathogen pathovar tomato ( ). Our transmission electron microscopy and scanning electron microscopy studies indicate that the mutant leaves have a thinner cuticular layer, defective stomata structure, and impaired cuticle ledge of stomata compared to the leaves of wild type plants. GC–MS analysis further revealed that the amount of cutin monomers was significantly reduced in mutant plants. Furthermore, the exogenous application of either of three plant hormones—salicylic acid, jasmonic acid, or abscisic acid—enhanced the cuticle formation in mutant leaves and the related defense responses to the bacterial infection. Thus, transport of cutin-related components by AtMIN7 may contribute to its impact on cuticle formation and related defense function.
机译:该角质层是植物空中组织的最外层,其与环境相互作用,保护植物免受水分损失和各种生物和非生物应激。 ADP核糖基化因子鸟嘌呤核苷酸交换因子蛋白(ARF-GEF)是囊泡贩运系统的关键组分。我们的研究发现了ATMIN7,ARF-GEF,对Cuclics形成和相关叶片表面防御对细菌病原体病毒番茄()至关重要。我们的透射电子显微镜和扫描电子显微镜研究表明,与野生型植物的叶子相比,突变叶具有较薄的有薄层层,缺陷的气孔结构,气孔晶体受损。 GC-MS分析进一步揭示了Cutin单体的量在突变植物中显着降低。此外,三种植物激素 - 水杨酸,茉莉酸或脱落酸的外源性应用 - 增强突变叶中的角质层形成和对细菌感染的相关防御反应。因此,ATMIN7的Cutin相关组分的运输可能有助于其对角质层形成和相关防御功能的影响。

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