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Drought Disrupts Auxin Localization in Abscission Zone and Modifies Cell Wall Structure Leading to Flower Separation in Yellow Lupine

机译:干旱扰乱脱落区中的养阴定位并改变细胞壁结构导致黄羽羽的花分离

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摘要

Drought causes the excessive abscission of flowers in yellow lupine, leading to yield loss and serious economic consequences in agriculture. The structure that determines the time of flower shedding is the abscission zone (AZ). Its functioning depends on the undisturbed auxin movement from the flower to the stem. However, little is known about the mechanism guiding cell–cell adhesion directly in an AZ under water deficit. Therefore, here, we seek a fuller understanding of drought-dependent reactions and check the hypothesis that water limitation in soil disturbs the natural auxin balance within the AZ and, in this way, modifies the cell wall structure, leading to flower separation. Our strategy combined microscopic, biochemical, and chromatography approaches. We show that drought affects indole-3-acetic acid (IAA) distribution and evokes cellular changes, indicating AZ activation and flower abortion. Drought action was manifested by the accumulation of proline in the AZ. Moreover, cell wall-related modifications in response to drought are associated with reorganization of methylated homogalacturonans (HG) in the AZ, and upregulation of pectin methylesterase (PME) and polygalacturonase (PG)—enzymes responsible for pectin remodeling. Another symptom of stress action is the accumulation of hemicelluloses. Our data provide new insights into cell wall remodeling events during drought-induced flower abscission, which is relevant to control plant production.
机译:干旱导致黄羽羽的鲜花过度脱落,导致农业造成损失和严重的经济后果。确定花脱落时间的结构是脱落区(AZ)。它的功能取决于从花到茎的未受干扰的植物素运动。然而,关于在水赤字下直接引导细胞 - 细胞粘附的机理粘附很少。因此,在这里,我们寻求更全面了解干旱依赖性反应,并检查土壤中水限制的假设在AZ中扰乱天然毒素平衡,并以这种方式改变细胞壁结构,导致花分离。我们的策略组合显微镜,生化和色谱方法。我们表明干旱影响吲哚-3-醋酸(IAA)分布并引发细胞变化,表明AZ激活和花卉流产。在AZ中脯氨酸的积累表现出干旱作用。此外,响应于干旱的细胞壁相关修饰与AZ中甲基化同源肌炎(Hg)的重组相关,以及果胶甲基酯酶(PME)的上调和负责果胶重塑的多糖酶(PG) - 酶。压力作用的另一个症状是半纤维素的积累。我们的数据在干旱诱导的花脱落期间对细胞壁改造事件提供了新的见解,这与控制植物生产相关。

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