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首页> 美国卫生研究院文献>Marine Drugs >Application of Six Detection Methods for Analysis of Paralytic Shellfish Toxins in Shellfish from Four Regions within Latin America
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Application of Six Detection Methods for Analysis of Paralytic Shellfish Toxins in Shellfish from Four Regions within Latin America

机译：六种检测方法在拉丁美洲四个地区分析贝类贝类血浆贝类毒素的应用

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摘要

With the move away from use of mouse bioassay (MBA) to test bivalve mollusc shellfish for paralytic shellfish poisoning (PSP) toxins, countries around the world are having to adopt non-animal-based alternatives that fulfil ethical and legal requirements. Various assays have been developed which have been subjected to single-laboratory and multi-laboratory validation studies, gaining acceptance as official methods of analysis and approval for use in some countries as official control testing methods. The majority of validation studies conducted to date do not, however, incorporate shellfish species sourced from Latin America. Consequently, this study sought to investigate the performance of five alternative PSP testing methods together with the MBA, comparing the PSP toxin data generated both qualitatively and quantitatively. The methods included a receptor binding assay (RBA), two liquid chromatography with fluorescence detection (LC-FLD) methods including both pre-column and post-column oxidation, liquid chromatography with tandem mass spectrometry (LC-MS/MS) and a commercial lateral flow assay (LFA) from Scotia. A total of three hundred and forty-nine shellfish samples from Argentina, Mexico, Chile and Uruguay were assessed. For the majority of samples, qualitative results compared well between methods. Good statistical correlations were demonstrated between the majority of quantitative results, with a notably excellent correlation between the current EU reference method using pre-column oxidation LC-FLD and LC-MS/MS. The LFA showed great potential for qualitative determination of PSP toxins, although the findings of high numbers of false-positive results and two false negatives highlighted that some caution is still needed when interpreting results. This study demonstrated that effective replacement methods are available for countries that no longer wish to use the MBA, but highlighted the importance of comparing toxin data from the replacement method using local shellfish species of concern before implementing new methods in official control testing programs.
机译：随着远离使用小鼠生物测定（MBA）来测试双腭贝类贝类（PSP）毒素，世界各国必须采用基于非动物的替代品，以满足道德和法律要求。已经制定了各种测定，这已经进行了单实验室和多实验室验证研究，获得了作为官方控制测试方法的官方控制和批准的官方分析和批准方法的接受。然而，迄今为止的大多数验证研究并未加入来自拉丁美洲的贝类物种。因此，该研究寻求研究五个替代PSP测试方法与MBA的性能，比较定性和定量生成的PSP毒素数据。该方法包括受体结合测定（RBA），两种液相色谱，其荧光检测（LC-FLD）方法包括柱子前和后柱氧化，液相色谱法与串联质谱（LC-MS / MS）和商业来自斯科舍省的侧向流动测定（LFA）。评估了来自阿根廷，墨西哥，智利和乌拉圭共有三百四十九十九个贝类样品。对于大多数样本，定性结果在方法之间进行了比较。在大多数定量结果之间证明了良好的统计相关性，目前欧盟参考方法使用预柱氧化LC-FLD和LC-MS / MS之间具有显着优异的相关性。 LFA表现出质量测定PSP毒素的巨大潜力，尽管大量的假阳性结果和两个假底片的发现强调，在解释结果时仍然需要一些小心。本研究表明，不再希望使用MBA的国家可以获得有效的更换方法，但突出了使用当地贝类物种在实施官方控制测试程序中的新方法之前将玉米数据与替代方法进行比较的重要性。

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