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Epigenetic Modifiers Affect the Bioactive Compounds Secreted by an Endophyte of the Tropical Plant

机译：表观遗传改性剂会影响热带植物的内心细胞分泌的生物活性化合物

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摘要

Seven endophytic fungi were isolated from the tropical medicinal plant Piper longum L. After preliminary screening, Phomopsis heveicola was selected for the epigenetic activation treatments. The antibacterial, antifungal, and antioxidant potentials of crude extracts obtained from the treatments (with and without epigenetic modifiers) were analyzed in vitro. The extracts inhibited growth of the human pathogens Pseudomonas aeruginosa, Shigella sonnei, Streptococcus pyogenes, and Salmonella typhi, as well as the phytopathogens Puccinia recondita, Rhizoctonia solani, Phytophthora infestans, and Botrytis cinerea. Furthermore, DPPH-scavenging activity was higher in valproic acid treated extracts. Volatile chemicals with known biological activities (measured with GC-MS/MS), were released in the valproic acid treatment. The antimicrobial potentials of the extracts were confirmed using MRM/MS analysis. The experiments revealed a new promising endophytic fungus, P. heveicola, to be utilized in biological plant protection and in biomedical applications.
机译：从热带药用植物吹笛器Longum L中分离出七个内生真菌。在初步筛选后，选择了Heveicola的表观遗传活化处理。在体外分析从处理中获得的粗提取物（具有和不含表述改性剂）的抗菌，抗真菌和抗氧化潜力。提取物抑制人病原体假单胞菌铜绿假单胞菌，志贺氏菌，链球菌的生长和沙门氏菌Typhi，以及植物病原体宫内节育脉，Rhizoctonia solani，植物细胞，嗜孢子虫和Botrytis cinerea。此外，丙甲酸处理过的提取物中DPPH-清除活性较高。在丙戊酸处理中释放具有已知生物活性的挥发性化学品（用GC-MS / MS测量）。使用MRM / MS分析确认提取物的抗微生物电位。实验揭示了一种新的有前途的内心真菌，P.Heveicola，用于生物植物保护和生物医学应用。

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