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The complete chloroplast genome sequence of Dendrobium thyrsiflorum (Orchidaceae) and its phylogenetic analysis

机译:Dendrobium thyrsiflorum(兰科)的完全叶绿体基因组序列及其系统发育分析

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摘要

Dendrobium thyrsiflorum Rchb.f., a native species to China, is widely used as an important garden flower and a traditional Chinese medicine. Herein, the complete chloroplast (cp) genome of D. thyrsiflorum was deciphered by high-throughput sequencing. The cp genome exhibited a typical quadripartite cycle of 151,686 bp in length, comprising of a pair of inverted repeats (IRa and IRb) of 26,293 bp which were intersected by a large single copy (LSC) region of 84,749 bp and a small single copy (SSC) region of 14,351 bp. A total of 126 genes were de novo assembled in this cp genome, including 78 protein genes, 40 tRNA genes and 8 rRNA genes. Among these genes, 86 genes (22 tRNAs and 64 coding genes) were single copy, the rest were two-copy genes, and the average of GC content of the whole genome is 37.55%. Phylogenetic trees showed that the D. thyrsiflorum was closely related to D. devonianum. This study provides molecular information for future evolution, genetic and molecular biology studies of Dendrobium.
机译:Dendrobium thyrsiflorum Rchb.f.,中国天然物种,被广泛用作重要的花园花和中药。这里,通过高通量测序破坏D.甲基甲基罗鲁姆的完整叶绿体(CP)基因组。 CP基因组长度表现出151,686bp的典型Quadriparte循环,其具有26,293bp的一对倒重复(IRA和IRB),其由84,749 bp的大单拷贝(LSC)区域和小单拷贝( SSC)14,351 BP的区域。在该CP基因组中,共组装了126个基因,包括78个蛋白质基因,40个TRNA基因和8 rRNA基因。在这些基因中,86个基因(22个TreNAS和64个编码基因)是单拷贝,其余的是两拷贝基因,整个基因组的GC含量的平均值为37.55%。系统发育树表明,D. Thyrsiflorum与D. devonianum密切相关。本研究为石斛的未来演化,遗传和分子生​​物学研究提供了分子信息。

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