• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The Journal of Biological Chemistry >Identification of Chromosomal HP0892-HP0893 Toxin-Antitoxin Proteins in Helicobacter pylori and Structural Elucidation of Their Protein-Protein Interaction
【2h】

Identification of Chromosomal HP0892-HP0893 Toxin-Antitoxin Proteins in Helicobacter pylori and Structural Elucidation of Their Protein-Protein Interaction

机译：幽门螺杆菌中染色体HP0892-HP0893毒素-抗毒素蛋白的鉴定及其蛋白-蛋白质相互作用的结构解析

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Bacterial chromosomal toxin-antitoxin (TA) systems have been proposed not only to play an important role in the stress response, but also to be associated with antibiotic resistance. Here, we identified the chromosomal HP0892-HP0893 TA proteins in the gastric pathogen, Helicobacter pylori, and structurally characterized their protein-protein interaction. Previously, HP0892 protein was suggested to be a putative TA toxin based on its structural similarity to other RelE family TA toxins. In this study, we demonstrated that HP0892 binds to HP0893 strongly with a stoichiometry of 1:1, and HP0892-HP0893 interaction occurs mainly between the N-terminal secondary structure elements of HP0892 and the C-terminal region of HP0893. HP0892 cleaved mRNA in vitro, preferentially at the 5′ end of A or G, and the RNase activity of HP0892 was inhibited by HP0893. In addition, heterologous expression of HP0892 in Escherichia coli cells led to cell growth arrest, and the cell toxicity of HP0892 was neutralized by co-expression with HP0893. From these results and a structural comparison with other TA toxins, it is concluded that HP0892 is a toxin with intrinsic RNase activity and HP0893 is an antitoxin against HP0892 from a TA system of H. pylori. It has been known that hp0893 gene and another TA antitoxin gene, hp0895, of H. pylori, are both genomic open reading frames that correspond to genes that are potentially expressed in response to interactions with the human gastric mucosa. Therefore, it is highly probable that TA systems of H. pylori are involved in virulence of H. pylori.
机译：已经提出细菌染色体毒素-抗毒素（TA）系统不仅在应激反应中起重要作用，而且与抗生素耐药性有关。在这里，我们确定了胃病原体幽门螺杆菌中的染色体HP0892-HP0893 TA蛋白，并在结构上表征了它们之间的蛋白-蛋白相互作用。以前，基于其与其他RelE家族TA毒素的结构相似性，HP0892蛋白被认为是公认的TA毒素。在这项研究中，我们证明了HP0892以1：1的化学计量比与HP0893牢固结合，并且HP0892-HP0893相互作用主要发生在HP0892的N末端二级结构元素和HP0893的C末端区域之间。 HP0892体外切割mRNA，优先在A或G的5'端，HP0893抑制HP0892的RNase活性。此外，HP0892在大肠杆菌细胞中的异源表达导致细胞生长停滞，并且通过与HP0893共表达中和了HP0892的细胞毒性。从这些结果以及与其他TA毒素的结构比较，可以得出结论，HP0892是具有固有RNase活性的毒素，HP0893是针对幽门螺杆菌TA系统的HP0892的抗毒素。已知幽门螺杆菌的hp0893基因和另一个TA抗毒素基因hp0895都是基因组开放阅读框，它们对应于响应于与人胃粘膜相互作用而潜在表达的基因。因此，幽门螺杆菌的TA系统很可能与幽门螺杆菌的毒力有关。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号