首页> 美国卫生研究院文献>International Journal of Molecular Sciences >G-Quadruplex in Gene Encoding Large Subunit of Plant RNA Polymerase II: A Billion-Year-Old Story
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G-Quadruplex in Gene Encoding Large Subunit of Plant RNA Polymerase II: A Billion-Year-Old Story

机译:G-quadruplex在编码植物RNA聚合酶II的大亚基的基因:十亿岁的故事

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摘要

G-quadruplexes have long been perceived as rare and physiologically unimportant nucleic acid structures. However, several studies have revealed their importance in molecular processes, suggesting their possible role in replication and gene expression regulation. Pathways involving G-quadruplexes are intensively studied, especially in the context of human diseases, while their involvement in gene expression regulation in plants remains largely unexplored. Here, we conducted a bioinformatic study and performed a complex circular dichroism measurement to identify a stable G-quadruplex in the gene RPB1, coding for the RNA polymerase II large subunit. We found that this G-quadruplex-forming locus is highly evolutionarily conserved amongst plants sensu lato (Archaeplastida) that share a common ancestor more than one billion years old. Finally, we discussed a new hypothesis regarding G-quadruplexes interacting with UV light in plants to potentially form an additional layer of the regulatory network.
机译:长期以来长期被认为是罕见的和生理学上不重要的核酸结构。然而,若干研究表明了它们在分子过程中的重要性,表明其在复制和基因表达调节中可能的作用。涉及G-QuadrupleSes的途径是集中研究的,特别是在人类疾病的背景下,它们参与植物中的基因表达调节仍然很大程度上是未开发的。在这里,我们进行了生物信息化研究,并进行了复杂的圆形二色性测量,以鉴定基因RPB1中的稳定G-Quadflex,编码RNA聚合酶II大亚基。我们发现,这种G-Quadruple的轨迹在植物Sensu Lato(Archaeplastida)之间具有高度进化的水平保守,这些祖先超过10亿岁以上的祖先。最后,我们讨论了关于与植物中的UV光相互作用的G-Quadruplecs的新假设,以潜在地形成监管网络的附加层。

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