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首页> 美国卫生研究院文献>International Journal of Environmental Research and Public Health >Cytotoxicity and Apoptosis Induced by Chenopodium ambrosioides L. Essential Oil in Human Normal Liver Cell Line L02 via the Endogenous Mitochondrial Pathway Rather Than the Endoplasmic Reticulum Stress
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Cytotoxicity and Apoptosis Induced by Chenopodium ambrosioides L. Essential Oil in Human Normal Liver Cell Line L02 via the Endogenous Mitochondrial Pathway Rather Than the Endoplasmic Reticulum Stress

机译：通过内源线粒体途径而非内质网应力通过内源性线粒体途径由内源性肝细胞系L02中的精油诱导的细胞毒性和凋亡。通过内源性线粒体途径而不是内质网胁迫

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Chenopodium ambrosioides L. (C. ambrosioides) has been used as dietary condiments and as traditional medicine in South America. The oil of Chenopodium ambrosioides L. (C. ambrosioides) can be used as a natural antioxidant in food processing. It also has analgesic, sedating, and deworming effects, and can be used along with the whole plant for its medical effects: decongestion, as an insecticide, and to offer menstruation pain relief. This study was conducted to investigate the cytotoxicity and apoptosis effects of an essential oil from C. ambrosioides in vitro. The cytotoxicity evaluation of the essential oil from C. ambrosioides on human normal liver cell line L02 was assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. AO/EB dual fluorescent staining assay and Annexin V-FITC were used for apoptosis analysis. The changes in mitochondrial membrane potential (MMP) were analyzed with 5,5,6,6′-tetrachloro-1,1,3,3,-tetraethyl-imidacarbocyanine iodide (JC-1) dye under a fluorescence microscope. The level of apoptosis related protein expression was quantified by Western blot. The L02 cells were treated with the essential oil from C. ambrosioides at 24, 48, and 72 h, and the IC50 values were 65.45, 58.03, and 35.47 μg/mL, respectively. The AO/EB staining showed that viable apoptotic cells, non-viable apoptotic cells, and non-viable non-apoptotic cells appeared among the L02 cells under the fluorescence microscope. Cell cycle arrest at the S phase and cell apoptosis increased through flow cytometry in the L02 cells treated with the essential oil. MMP decreased in a concentration-dependent manner, as seen through JC-1 staining under the fluorescence microscope. In the L02 cells as shown by Western blot and qPCR, the amount of the apoptosis-related proteins and the mRNA expression levels of cytochrome C, Bax, Caspase-9, and Caspase-3 increased, Bcl-2 decreased, and Caspase-12, which is expressed in the endoplasmic reticulum, showed no obvious changes in protein amount or mRNA expression level. The essential oil form C. ambrosioides had a cytotoxic effect on L02 cells. It could inhibit L02 cell proliferation, arrest the cell cycle at the S phase, and induce L02 cell apoptosis through the endogenous mitochondrial pathway.
机译：辛泛酸铵胺酰亚胺L.（C.Ambrosioides）已被用作膳食调味品，并作为南美洲的传统医学。辛博二核苷酸的油石油（C.Ambosioides）可用作食品加工的天然抗氧化剂。它还具有镇痛，镇静和驱虫作用，可与整个植物一起使用，以获得其医疗效果：减肥，作为杀虫剂，并提供月经疼痛缓解。进行该研究以研究在体外C.氨基醚精油的细胞毒性和凋亡作用。通过3-（4,5-二甲基-2-噻唑基）-2,5-二苯基-2- H-四唑（MTT）评估来自人正常肝细胞系L02上的C.氨基脲的细胞毒性评价。测定。 AO / EB双荧光染色测定和膜蛋白V-FITC用于凋亡分析。用5,5,6,6'-四氯-1,1,3,3， - 在荧光显微镜下用5,5,6,6'-四氯-1,1,3,3，-Tethyl-吡酰碳碳糖胺碘化物（JC-1）染料分析线粒体膜电位（MMP）的变化。通过Western印迹量化凋亡相关蛋白表达水平。将L02细胞用来自C.在24,48和72小时的氨基脲的精油处理，IC 50值分别为65.45,58.03和35.47μg/ mL。 AO / EB染色表明，在荧光显微镜下的L02细胞中出现了可行的凋亡细胞，不行凋亡细胞和不活性的非凋亡细胞。在S期和细胞凋亡的细胞周期停滞通过用精油处理的L02细胞中的流式细胞术增加。 MMP以浓度依赖性方式减少，如荧光显微镜下通过JC-1染色所见。在蛋白质印迹和QPCR所示的L02细胞中，细胞凋亡相关蛋白的量和细胞色素C，Bax，Caspase-9和Caspase-3的mRNA表达水平增加，Bcl-2降低，以及Caspase-12 ，其在内质网中表达，表明蛋白质量或mRNA表达水平没有明显的变化。精油形式C.Ambrosioides对L02细胞具有细胞毒性作用。它可以抑制L02细胞增殖，在S期抑制细胞周期，并通过内源线粒体途径诱导L02细胞凋亡。

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