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首页> 美国卫生研究院文献>Journal of Bacteriology >Light Effects in Yeast: Evidence for Participation of Cytochromes in Photoinhibition of Growth and Transport in Saccharomyces cerevisiae Cultured at Low Temperatures
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Light Effects in Yeast: Evidence for Participation of Cytochromes in Photoinhibition of Growth and Transport in Saccharomyces cerevisiae Cultured at Low Temperatures

机译：酵母中的光效应：低温培养的酿酒酵母中细胞色素参与光抑制生长和运输的证据。

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Visible light of moderate intensity inhibits growth, respiration, protein synthesis, and membrane transport in bakers' yeast and has a deleterious effect on membrane integrity. The results of this study indicate that these effects require the presence of cytochromes b and a/a3. The light sensitivities of growth rate and [14C]histidine uptake in wild-type rho+ Y185 and D225-5A strains of Saccharomyces cerevisiae were compared with those in a variety of mutants lacking cytochrome b or a/a3 or both; a close correlation was found between the presence of these respiratory pigments and photosensitivity. Thus, strain TL5-3C, a nuclear petite lacking cytochromes b, a, and a3, was resistant to light; strain GL5-6A, another nuclear petite having reduced amounts of cytochromes a and a3, was partially resistant; strains MB127-20C and MB1-6C, nuclear petites lacking only cytochrome b, were also only partially resistant to light; whereas mutants containing all three cytochromes but having their respiratory chain either nonfunctional (strain ZK3-6B) or uncoupled (strain 18-27/t12) were fully sensitive to light. Finally, an equal-energy, broad-band action spectrum for the light inhibition of growth and transport indicated that blue light (408 nm) was most effective; these wavelengths correspond to the Soret region of the cytochrome absorption spectrum. The results suggest, therefore, that the yeast cytochromes b, a, and a3 are the primary photoreceptors for the inhibitory effects of light and, perhaps, for other processes, such as the entrainment of biological rhythms in this species.
机译：中等强度的可见光会抑制面包酵母中的生长，呼吸，蛋白质合成和膜运输，并对膜的完整性产生有害影响。这项研究的结果表明，这些作用需要细胞色素b和a / a3的存在。比较了酿酒酵母野生型rho + Y185和D225-5A菌株的生长速率和[ 14 C]组氨酸吸收的光敏性，并比较了缺少细胞色素b或a / a3或两者都不存在的突变体；这些呼吸色素的存在与光敏性之间存在密切的相关性。因此，菌株TL5-3C（一种缺少细胞色素b，a和a3的核娇小）对光具有抵抗力。 GL5-6A菌株是另一种具有减少的细胞色素a和a3含量的核娇小菌，具有部分抗性。 MB127-20C和MB1-6C菌株，仅缺少细胞色素b的核小体，也仅部分抗光。而包含所有三种细胞色素但呼吸链不起作用（菌株ZK3-6B）或未偶联（菌株18-27 / t12）的突变体对光完全敏感。最后，用等能量的宽带作用谱来抑制生长和运输的光，表明蓝光（408 nm）最有效。这些波长对应于细胞色素吸收光谱的Soret区。因此，结果表明，酵母细胞色素b，a和a3是光抑制作用以及其他过程（例如在该物种中夹带生物节律）的主要光感受器。

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