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Cloning and characterization of the ponA gene encoding penicillin-binding protein 1 from Neisseria gonorrhoeae and Neisseria meningitidis.

机译：淋病奈瑟氏球菌和脑膜炎奈瑟氏球菌青霉素结合蛋白1 ponA基因的克隆与鉴定。

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摘要

The ponA gene encoding penicillin-binding protein 1 (PBP 1) from Neisseria gonorrhoeae was cloned by a reverse genetic approach. PBP 1 was purified from solubilized membranes of penicillin-susceptible strain FA19 by covalent ampicillin affinity chromatography and used to obtain an NH2-terminal amino acid sequence. A degenerate oligonucleotide based on this protein sequence and a highly degenerate oligonucleotide based on a conserved amino acid motif found in all class A high-molecular-mass PBPs were used to isolate the PBP 1 gene (ponA). The ponA gene encodes a protein containing all of the conserved sequence motifs found in class A PBPs, and expression of the gene in Escherichia coli resulted in the appearance of a new PBP that comigrated with PBP 1 purified from N. gonorrhoeae. A comparison of the gonococcal ponA gene to its homolog isolated from Neisseria meningitidis revealed a high degree of identity between the two gene products, with the greatest variability found at the carboxy terminus of the two deduced PBP 1 protein sequences.

机译：通过反向遗传方法克隆了淋病奈瑟氏球菌编码青霉素结合蛋白1（PBP 1）的ponA基因。通过共价氨苄青霉素亲和色谱法从青霉素敏感性菌株FA19的增溶膜中纯化PBP 1，并用于获得NH 2末端氨基酸序列。使用基于该蛋白质序列的简并寡核苷酸和基于所有A类高分子PBP中发现的保守氨基酸基序的高度简并寡核苷酸来分离PBP 1基因（ponA）。 ponA基因编码一种蛋白质，该蛋白质包含在A类PBP中发现的所有保守序列基序，该基因在大肠杆菌中的表达导致出现新的PBP，该新PBP与从淋病奈瑟氏球菌中纯化的PBP 1相对应。淋球菌ponA基因与其从脑膜炎奈瑟氏球菌分离的同系物的比较表明，这两个基因产物之间具有高度同一性，在两个推导的PBP 1蛋白序列的羧基末端发现最大的变异性。

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期刊名称 Journal of Bacteriology
作者
P A Ropp; R A Nicholas;
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作者单位

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年(卷),期 1997(179),8
年度 1997
页码 2783–2787
总页数 5
原文格式 PDF
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中图分类微生物学;
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专利

1. Cloning and characterization of the ponA gene encoding penicillin-binding protein 1 from Neisseria gonorrhoeae and Neisseria meningitidis. [J] . P A Ropp, R A Nicholas Journal of bacteriology . 1997,第8期

机译：淋病奈瑟氏球菌和脑膜炎奈瑟氏球菌青霉素结合蛋白1 ponA基因的克隆与鉴定。
2. Mutations in ponA, the gene encoding penicillin-binding protein 1, and a novel locus, penC, are required for high-level chromosomally mediated penicillin resistance in Neisseria gonorrhoeae. [J] . Ropp PatriciaA, Hu Mei, Olesky Melanie, Antimicrobial agents and chemotherapy. . 2002,第3期

机译：淋病奈瑟氏球菌的高水平染色体介导的青霉素抗性需要ponA（编码青霉素结合蛋白1的基因）和新基因座penC的突变。
3. Cloning, sequencing, and characterization of the gene encoding FrpB, a major iron-regulated, outer membrane protein of Neisseria gonorrhoeae. [J] . M Beucher, P F Sparling Journal of bacteriology . 1995,第8期

机译：编码FrpB（淋病奈瑟氏球菌的主要铁调节性外膜蛋白）的基因的克隆，测序和表征。
4. Cloning and characterization of three homoeologous genes encoding PDI (Protein Disulfide Isomerase) and their promoter sequences in wheat. [C] . M. Ciaffi, A. Zanotti, A. R. Paolacci, International Wheat Genetics Symposium . 2003

机译：小麦PDI（蛋白质二硫化物异构酶）及其启动子序列的三种同源基因的克隆与表征。
5. Characterization of the gene encoding Omp85, a novel outer membrane protein of Neisseria gonorrhoeae and introduction of genes into Neisseria gonorrhoeae. [D] . Reschke, Dennis Karl. 1998

机译：编码淋病奈瑟氏球菌的新型外膜蛋白Omp85的基因的鉴定，并将基因导入淋病奈瑟氏球菌。
6. Mutations in ponA the Gene Encoding Penicillin-Binding Protein 1 and a Novel Locus penC Are Required for High-Level Chromosomally Mediated Penicillin Resistance in Neisseria gonorrhoeae [O] . Patricia A. Ropp, Mei Hu, Melanie Olesky, 2002

机译：淋病奈瑟菌的高水平染色体介导的青霉素抗性需要ponA编码青霉素结合蛋白1的基因和新型基因penC的突变。
7. Cloning and characterization of the ponA gene encoding penicillin-binding protein 1 from Neisseria gonorrhoeae and Neisseria meningitidis. [O] . Ropp, P A, Nicholas, R A 1997

机译：淋病奈瑟氏球菌和脑膜炎奈瑟氏球菌青霉素结合蛋白1 ponA基因的克隆与鉴定。
8. Heterogeneity of Molecular Size and Antigenic Expression within Lipooligosaccharides of Individual Strains of Neisseria gonorrhoeae and Neisseria meningitidis. [R] . Schneider, H., Hale, T. L., Zollinger, W. D., 1984

机译：淋病奈瑟菌和脑膜炎奈瑟球菌个别低密度脂蛋白的分子大小和抗原表达的异质性。

Cloning and characterization of the ponA gene encoding penicillin-binding protein 1 from Neisseria gonorrhoeae and Neisseria meningitidis.

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