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首页> 美国卫生研究院文献>Journal of Bacteriology >The GAF-Like-Domain-Containing Transcriptional Regulator DfdR Is a Sensor Protein for Dibenzofuran and Several Hydrophobic Aromatic Compounds
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The GAF-Like-Domain-Containing Transcriptional Regulator DfdR Is a Sensor Protein for Dibenzofuran and Several Hydrophobic Aromatic Compounds

机译：包含GAF样域的转录调节子DfdR是二苯并呋喃和几种疏水性芳香族化合物的传感蛋白。

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Dibenzofuran (DF) is one of the dioxin carbon skeletal compounds used as a model to study the microbial degradation of dioxins. This study analyzed the transcriptional regulation of the DF dioxygenase genes dfdA1 to dfdA4 in the DF-utilizing actinomycetes Rhodococcus sp. strain YK2 and Terrabacter sp. strain YK3. An open reading frame designated dfdR was detected downstream of the dfdC genes. The C-terminal part of the DfdR amino acid sequence has high levels of similarity to several LuxR-type DNA binding helix-turn-helix domains, and a GAF domain sequence in the central part was detected by a domain search analysis. A derivative of YK2 with dfdR disrupted was not able to utilize DF and did not exhibit DF-dependent dfdA1 transcriptional induction ability, and these dysfunctions were compensated for by introduction of dfdR. Promoter analysis of dfdA1 in Rhodococcus strains indicated that activation of the dfdA1 promoter (PdfdA1) was dependent on dfdR and DF and not on a metabolite of the DF pathway. The cell extract of a Rhodococcus strain that heterologously expressed DfdR showed electrophoretic mobility shift (EMS) activity for the PdfdA1 DNA fragment in a DF-dependent manner. In addition, PdfdA1 activation and EMS activity were observed with hydrophobic aromatic compounds comprising two or more aromatic rings, suggesting that DfdR has broad effector molecule specificity for several hydrophobic aromatic compounds.
机译：二苯并呋喃（DF）是一种二恶英碳骨架化合物，用作研究二恶英的微生物降解的模型。本研究分析了利用DF的放线菌Rhodococcus sp中DF双加氧酶基因dfdA1至dfdA4的转录调控。 YK2菌株和Terrabacter sp。 YK3株。在dfdC基因的下游检测到一个开放阅读框dfdR。 DfdR氨基酸序列的C端部分与几个LuxR型DNA结合的螺旋-螺旋-螺旋结构域具有高度相似性，并且通过结构域搜索分析检测了中央部分的GAF结构域序列。 dfdR被破坏的YK2衍生物不能利用DF，也没有表现出DF依赖的dfdA1转录诱导能力，这些功能障碍可以通过引入dfdR来弥补。对红球菌菌株中dfdA1的启动子分析表明，dfdA1启动子（PdfdA1）的激活取决于dfdR和DF，而不依赖于DF途径的代谢产物。异源表达DfdR的红球菌菌株的细胞提取物以DF依赖的方式显示PdfdA1 DNA片段的电泳迁移率（EMS）活性。此外，在包含两个或多个芳香环的疏水性芳香族化合物中观察到P 活化和EMS活性，表明DfdR对几种疏水性芳香族化合物具有广泛的效应分子特异性。

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