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Overlapping Alternative Sigma Factor Regulons in the Response to Singlet Oxygen in Rhodobacter sphaeroides

机译:重叠球菌红球菌对单线态氧的响应中的替代西格玛因子调节子。

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摘要

Organisms performing photosynthesis in the presence of oxygen have to cope with the formation of highly reactive singlet oxygen (1O2) and need to mount an adaptive response to photooxidative stress. Here we show that the alternative sigma factors RpoHI and RpoHII are both involved in the 1O2 response and in the heat stress response in Rhodobacter sphaeroides. We propose RpoHII to be the major player in the 1O2 response, whereas RpoHI is more important for the heat stress response. Mapping of the 5′ ends of RpoHII- and also RpoHI/RpoHII-dependent transcripts revealed clear differences in the −10 regions of the putative promoter sequences. By using bioinformatic tools, we extended the RpoHII regulon, which includes genes induced by 1O2 exposure. These genes encode proteins which are, e.g., involved in methionine sulfoxide reduction and in maintaining the quinone pool. Furthermore, we identified small RNAs which depend on RpoHI and RpoHII and are likely to contribute to the defense against photooxidative stress and heat stress.
机译:在氧气存在下进行光合作用的生物必须应对高反应性单线态氧气( 1 O2)的形成,并需要对光氧化应激做出适应性响应。在这里,我们显示替代的sigma因子RpoHI和RpoHII都参与了球形球形红细菌的 1 O2响应和热应激响应。我们建议RpoHII是 1 O2反应的主要参与者,而RpoHI对于热应激反应更为重要。 RpoHII-和RpoHI / RpoHII依赖的转录本的5'端的映射揭示了推定的启动子序列的-10区明显不同。通过使用生物信息学工具,我们扩展了RpoHII regulon,其中包括由 1 O2暴露诱导的基因。这些基因编码例如参与蛋氨酸亚砜还原和维持醌库的蛋白质。此外,我们鉴定了依赖RpoHI和RpoHII的小RNA,它们可能有助于防御光氧化应激和热应激。

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