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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >A Simple and Effective Mass Spectrometric Approach to Identify the Adulteration of the Mediterranean Diet Component Extra-Virgin Olive Oil with Corn Oil
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A Simple and Effective Mass Spectrometric Approach to Identify the Adulteration of the Mediterranean Diet Component Extra-Virgin Olive Oil with Corn Oil

机译：一种简单有效的质谱方法来鉴定地中海饮食成分特级初榨橄榄油与玉米油的掺假

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摘要

Extra virgin olive oil (EVOO) with its nutraceutical characteristics substantially contributes as a major nutrient to the health benefit of the Mediterranean diet. Unfortunately, the adulteration of EVOO with less expensive oils (e.g., peanut and corn oils), has become one of the biggest source of agricultural fraud in the European Union, with important health implications for consumers, mainly due to the introduction of seed oil-derived allergens causing, especially in children, severe food allergy phenomena. In this regard, revealing adulterations of EVOO is of fundamental importance for health care and prevention reasons, especially in children. To this aim, effective analytical methods to assess EVOO purity are necessary. Here, we propose a simple, rapid, robust and very sensitive method for non-specialized mass spectrometric laboratory, based on the matrix-assisted laser desorption/ionization mass spectrometry (MALDI-TOF MS) coupled to unsupervised hierarchical clustering (UHC), principal component (PCA) and Pearson’s correlation analyses, to reveal corn oil (CO) adulterations in EVOO at very low levels (down to 0.5%).
机译：具有营养保健特性的特级初榨橄榄油（EVOO）可以作为一种主要营养素，对地中海饮食的健康有益。不幸的是，EVOO掺假价格较低的油（例如花生油和玉米油）已成为欧盟农业欺诈的最大来源之一，对消费者的健康产生重要影响，主要是由于引入了种子油，衍生的过敏原，尤其是在儿童中，引起严重的食物过敏现象。在这方面，出于医疗保健和预防的原因，尤其是在儿童中，揭示EVOO的掺假至关重要。为此，需要有效的分析方法来评估EVOO纯度。在此，我们基于基质辅助激光解吸/电离质谱（MALDI-TOF MS）结合无监督分级聚类（UHC），为非专业质谱实验室提出了一种简单，快速，鲁棒且非常灵敏的方法成分（PCA）和Pearson相关分析，发现EVOO中玉米油（CO）的掺假水平非常低（低至0.5％）。

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