class='head no_bottom_margin' id='sec1title'>Int'/> Septins Recognize and Entrap Dividing Bacterial Cells for Delivery to Lysosomes
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Elsevier Sponsored Documents >Septins Recognize and Entrap Dividing Bacterial Cells for Delivery to Lysosomes
【2h】

Septins Recognize and Entrap Dividing Bacterial Cells for Delivery to Lysosomes

机译：Septins识别并捕获分离的细菌细胞以递送至溶酶体

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

class="head no_bottom_margin" id="sec1title">IntroductionSeptins are highly conserved filament-forming proteins that play key roles in eukaryotic cell division (, ). Originally discovered for their role in division of budding yeast (), recent work has shown that septins also play a role in division of mitochondria (, ). In humans, the 13 septins are classified into four homology groups (SEPT2, SEPT3, SEPT6, and SEPT7) where septins from different groups form hetero-oligomers and assemble into non-polar filaments, which can form higher-order structures including bundles and rings (). Septins have recently been shown to recognize areas of the plasma membrane presenting micron-scale curvature, including the cytokinetic furrow and phagocytic cup (). Although viewed as a fundamental property of the septin cytoskeleton (), signals responsible for septin recruitment to membrane, and the precise role of septin-membrane interactions, remain poorly understood.Shigella flexneri is taxonomically indistinguishable from Escherichia coli, but possesses a virulence plasmid encoding a type III secretion system (T3SS) that enables host cell invasion (). Following invasion, Shigella escapes from the phagosome to proliferate in the cytosol and polymerize actin tails for cell-to-cell spread (). To defend against Shigella invasion, host cells use a variety of mechanisms, including autophagy (), guanylate-binding proteins (GBPs) (, ), and septin-mediated cellular immunity (). To prevent bacterial dissemination, septins entrap actin-polymerizing bacteria in ∼1-μm (diameter) cage-like structures (). It has been shown that ∼50% of entrapped bacteria are metabolically inactive (href="#bib36" rid="bib36" class=" bibr popnode">Sirianni et al., 2016), but their fate is mostly unknown.The eukaryotic cytoskeleton is well known to rearrange during infection and play a crucial role in host-microbe interactions (href="#bib13" rid="bib13" class=" bibr popnode">Haglund and Welch, 2011). Components of the cytoskeleton mediate cellular immunity by enabling bacterial detection and mobilizing antibacterial mechanisms (href="#bib24" rid="bib24" class=" bibr popnode">Mostowy and Shenoy, 2015). Despite the septin cage representing an important link between the cytoskeleton and cellular immunity, we lack fundamental insights into how septins recognize bacteria for cage entrapment. Here, we discover that septin recognition of membrane curvature and growth during bacterial cell division is an unsuspected mechanism used by the host cell to defend against invasive pathogens.
机译：<！-fig ft0-> <！-fig @ position =“ anchor” mode =文章f4-> <！-fig mode =“ anchred” f5-> <！-fig / graphic | fig / alternatives / graphic mode =“ anchored” m1-> class =“ head no_bottom_margin” id =“ sec1title”>简介 Septins是高度保守的长丝形成蛋白，在真核细胞分裂中起关键作用（， ）。最初是由于它们在发芽酵母中的作用而发现的，最近的研究表明，Septin在线粒体的分裂中也起了作用。在人类中，这13种Septins被分为四个同源组（SEPT2，SEPT3，SEPT6和SEPT7），其中来自不同组的Septins形成杂合寡聚体并组装成非极性细丝，可以形成包括束和环的更高阶结构（）。最近显示，Septins可识别呈微米级曲率的质膜区域，包括细胞动力学沟槽和吞噬杯。尽管被认为是Septin细胞骨架的基本特性，但对于Septin募集到膜以及Septin与膜相互作用的确切作用的信号仍然知之甚少。弗氏志贺氏菌与大肠埃希菌在分类学上没有区别，但具有编码质粒的毒力质粒一种III型分泌系统（T3SS），可以使宿主细胞入侵（）。侵袭后，志贺氏菌从吞噬体中逃逸，在胞质溶胶中增殖并聚合肌动蛋白尾巴，从而在细胞间扩散（图）。为了防御志贺氏菌入侵，宿主细胞使用了多种机制，包括自噬（），鸟苷酸结合蛋白（GBP）（）和Septin介导的细胞免疫（）。为了防止细菌传播，隔膜将诱集肌动蛋白的细菌捕获在约1微米（直径）的笼状结构中（）。研究表明，约有50％的被捕获细菌没有代谢活性（href="#bib36" rid="bib36" class=" bibr popnode"> Sirianni et al。，2016 ），但它们的命运众所周知，真核细胞骨架在感染过程中会重新排列，并在宿主-微生物相互作用中起关键作用（href="#bib13" rid="bib13" class=" bibr popnode"> Haglund and Welch，2011 ）。细胞骨架的成分通过实现细菌检测和调动抗菌机制来介导细胞免疫（href="#bib24" rid="bib24" class=" bibr popnode"> Mostowy and Shenoy，2015 ）。尽管Septin笼代表了细胞骨架与细胞免疫之间的重要联系，但我们缺乏Septin如何识别细菌捕获笼子的基本见解。在这里，我们发现细菌细胞分裂过程中隔膜弯曲对膜曲率和生长的识别是宿主细胞用来防御入侵性病原体的不可思议的机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号