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首页> 美国卫生研究院文献>Elsevier Sponsored Documents >Ion mobility spectrometry combined with ultra performance liquid chromatography/mass spectrometry for metabolic phenotyping of urine: Effects of column length gradient duration and ion mobility spectrometry on metabolite detection
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Ion mobility spectrometry combined with ultra performance liquid chromatography/mass spectrometry for metabolic phenotyping of urine: Effects of column length gradient duration and ion mobility spectrometry on metabolite detection

机译：离子淌度光谱与超高效液相色谱/质谱联用进行尿液代谢表型分析：柱长梯度持续时间和离子淌度光谱对代谢物检测的影响

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摘要

The need for rapid and efficient high throughput metabolic phenotyping (metabotyping) in metabolomic/metabonomic studies often requires compromises to be made between analytical speed and metabolome coverage. Here the effect of column length (150, 75 and 30 mm) and gradient duration (15, 7.5 and 3 min respectively) on the number of features detected when untargeted metabolic profiling of human urine using reversed-phase gradient ultra performance chromatography with, and without, ion mobility spectrometry, has been examined. As would be expected, reducing column length from 150 to 30 mm, and gradient duration, from 15 to 3 min, resulted in a reduction in peak capacity from 311 to 63 and a similar reduction in the number of features detected from over ca. 16,000 to ca. 6500. Under the same chromatographic conditions employing UPLC/IMS/MS to provide an additional orthogonal separation resulted in an increase in the number of MS features detected to nearly 20,000 and ca. 7500 for the 150 mm and the 30 mm columns respectively. Based on this limited study the potential of LC/IMS/MS as a tool for improving throughput and increasing metabolome coverage clearly merits further in depth study.
机译：代谢组学/代谢组学研究中对快速有效的高通量代谢表型（代谢型）的需求通常需要在分析速度和代谢组学覆盖率之间做出折衷。当使用反相梯度超高效色谱对人尿进行非目标代谢谱分析时，柱长（150、75和30mm）和梯度持续时间（分别为15、7.5和3min）和梯度持续时间对检测到的特征数量的影响在没有离子迁移谱的情况下，已经进行了检查。如预期的那样，将色谱柱长度从150 mm减少到30 mm，将梯度持续时间从15 min减少到3 min，导致峰容量从311减少到63，并且从大约1200到1200毫安时检测到的特征数量也减少了16,000至6500。在相同的色谱条件下，采用UPLC / IMS / MS提供额外的正交分离，导致检测到的MS特征数增加到将近20,000和ca。 150毫米和30毫米色谱柱分别为7500。基于这项有限的研究，LC / IMS / MS作为提高通量和增加代谢组学覆盖率的工具的潜力显然值得进一步研究。

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