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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Molecular epidemiology of Corynebacterium diphtheriae from northwestern Russia and surrounding countries studied by using ribotyping and pulsed-field gel electrophoresis.
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Molecular epidemiology of Corynebacterium diphtheriae from northwestern Russia and surrounding countries studied by using ribotyping and pulsed-field gel electrophoresis.

机译：俄罗斯西北和周边国家白喉棒状杆菌的分子流行病学研究采用核糖分型法和脉冲场凝胶电泳技术。

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摘要

A selection of 100 Corynebacterium diphtheriae isolates from asymptomatic carriers and clinical cases from five regions in northwestern Russia were examined. Six additional isolates from patients in Finland and Estonia with epidemiological links to Russia were also examined. All isolates were characterized by biotyping, toxigenicity testing, ribotyping, and pulsed-field gel electrophoresis (PFGE). Hybridization of genomic DNA digested with BstEII revealed five ribotype patterns among the biotype gravis isolates (G1 through G5) and two patterns among the biotype mitis isolates (M1 and M2). PFGE using SfiI was not able to distinguish between ribotypes G1, G2, and G4. The predominant ribotype pattern, G1, found in cases of disease in all the areas studied, appears to be disseminating, in view of the isolates received from imported cases in Finland and Estonia. Among the 106 isolates examined, 68 produced pattern G1 and 24 produced pattern M1. Most of the M1 isolates were from the Leningrad Oblast region. Distinct ribotypes such as G2, G3, G4, G5, and M2 could represent endemic disease.
机译：从无症状携带者中选择了100种白喉棒状杆菌分离株，并研究了俄罗斯西北部五个地区的临床病例。还检查了芬兰和爱沙尼亚与俄罗斯有流行病学联系的另外六个分离株。所有分离株均通过生物分型，毒理学测试，核糖分型和脉冲场凝胶电泳（PFGE）进行表征。用BstEII消化的基因组DNA杂交显示，在生物型重症病毒分离株（G1至G5）中有5个核糖型模式，在生物型Mitis分离株（M1和M2）中有2个模式。使用SfiI的PFGE无法区分核糖型G1，G2和G4。鉴于从芬兰和爱沙尼亚的进口病例中分离到的分离株，在所有研究区域的疾病病例中发现的主要核糖型模式G1似乎正在传播。在检查的106个分离物中，有68个产生了模式G1，有24个产生了模式M1。大多数M1分离株来自列宁格勒州。诸如G2，G3，G4，G5和M2之类的独特核糖型可能代表地方病。

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