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首页> 美国卫生研究院文献>PLoS Clinical Trials >Genome-wide association mapping in winter barley for grain yield and culm cell wall polymer content using the high-throughput CoMPP technique
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Genome-wide association mapping in winter barley for grain yield and culm cell wall polymer content using the high-throughput CoMPP technique

机译：使用高通量CoMPP技术对冬小麦进行全基因组关联图谱分析，以了解谷物产量和茎细胞壁聚合物含量

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A collection of 112 winter barley varieties (Hordeum vulgare L.) was grown in the field for two years (2008/09 and 2009/10) in northern Italy and grain and straw yields recorded. In the first year of the trial, a severe attack of barley yellow mosaic virus (BaYMV) strongly influenced final performances with an average reduction of ~ 50% for grain and straw harvested in comparison to the second year. The genetic determination (GD) for grain yield was 0.49 and 0.70, for the two years respectively, and for straw yield GD was low in 2009 (0.09) and higher in 2010 (0.29). Cell wall polymers in culms were quantified by means of the monoclonal antibodies LM6, LM11, JIM13 and BS-400-3 and the carbohydrate-binding module CBM3a using the high-throughput CoMPP technique. Of these, LM6, which detects arabinan components, showed a relatively high GD in both years and a significantly negative correlation with grain yield (GYLD). Overall, heritability (H2) was calculated for GYLD, LM6 and JIM and resulted to be 0.42, 0.32 and 0.20, respectively. A total of 4,976 SNPs from the 9K iSelect array were used in the study for the analysis of population structure, linkage disequilibrium (LD) and genome-wide association study (GWAS). Marker-trait associations (MTA) were analyzed for grain yield and cell wall determination by LM6 and JIM13 as these were the traits showing significant correlations between the years. A single QTL for GYLD containing three MTAs was found on chromosome 3H located close to the Hv-eIF4E gene, which is known to regulate resistance to BaYMV. Subsequently the QTL was shown to be tightly linked to rym4, a locus for resistance to the virus. GWAs on arabinans quantified by LM6 resulted in the identification of major QTLs closely located on 3H and hypotheses regarding putative candidate genes were formulated through the study of gene expression levels based on bioinformatics tools.
机译：在意大利北部的田间种植了112种冬季大麦品种（Hordeum vulgare L.）两年（2008/09和2009/10），并记录了谷物和稻草的单产。在试验的第一年，大麦黄花叶病毒（BaYMV）的严重侵袭极大地影响了最终性能，与第二年相比，收获的谷物和稻草平均降低了约50％。两年的谷物产量遗传测定（GD）分别为0.49和0.70，而稻草产量的遗传测定GD在2009年较低（0.09），在2010年较高（0.29）。使用高通量CoMPP技术，通过单克隆抗体LM6，LM11，JIM13和BS-400-3和碳水化合物结合模块CBM3a对茎中的细胞壁聚合物进行定量。其中，检测阿拉伯聚糖成分的LM6在这两年中均显示出较高的GD，并且与谷物产量（GYLD）呈显着负相关。总体而言，计算得出的GYLD，LM6和JIM的遗传力（H 2 ）分别为0.42、0.32和0.20。该研究使用了来自9K iSelect阵列的总共4,976个SNP，用于分析种群结构，连锁不平衡（LD）和全基因组关联研究（GWAS）。通过LM6和JIM13对标记性状关联（MTA）的谷物产量和细胞壁测定进行了分析，因为这些性状表现出年份之间的显着相关性。在靠近Hv-eIF4E基因的3H染色体上发现了一个包含三个MTA的GYLD单个QTL，已知该基因可调节对BaYMV的抗性。随后，表明QTL与rym4紧密相连，而rym4是对该病毒产生抗药性的基因座。通过LM6定量的阿拉伯聚糖上的GWAs鉴定出了紧靠3H的主要QTL，并通过基于生物信息学工具的基因表达水平研究，提出了有关推定候选基因的假设。

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