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首页> 美国卫生研究院文献>Springer Open Choice >Effects of Different Selenium Levels on Gene Expression of a Subset of Selenoproteins and Antioxidative Capacity in Mice
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Effects of Different Selenium Levels on Gene Expression of a Subset of Selenoproteins and Antioxidative Capacity in Mice

机译：不同硒水平对小鼠硒蛋白亚基基因表达和抗氧化能力的影响

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This study aimed to evaluate how excess selenium induces oxidative stress by determining antioxidant enzyme activity and changes in expression of selected selenoproteins in mice. BALB/c mice (n = 20 per group) were fed a diet containing 0.045 (Se-marginal), 0.1 (Se-adequate), 0.4 (Se-supernutrition), or 0.8 (Se-excess) mg Se/kg. Gene expression was quantified in RNA samples extracted from the liver, kidney, and testis by real-time quantitative reverse transcription-polymerase chain reaction. We found that glutathione peroxidase (GPx) and catalase activities decreased in livers of mice fed the marginal or excess dose of Se as compared to those in the Se-adequate group. Additionally, superoxide dismutase and glutathione reductase activities were significantly reduced only in mice fed the excess Se diet, compared to animals on the adequate Se diet. Se-supernutrition had no effect on hepatic mRNA levels of GPx isoforms 1 and 4 (GPx1 and GPx4), down-regulated GPx isoform 3 (GPx3), and upregulated selenoprotein W (SelW) mRNA expression. The excess Se diet led to decreased hepatic mRNA levels of GPx1, GPx3 and GPx4 but no change in testicular mRNA levels of GPx1, GPx3 or SelW. Dietary Se had no effect on testicular mRNA levels of GPx4. Thus, our results suggest that Se exposure can reduce hepatic antioxidant capacity and cause liver dysfunction. Dietary Se was found to differentially regulate mRNA levels of the GPx family or SelW, depending on exposure. Therefore, these genes may play a role in the toxicity associated with Se.
机译：这项研究旨在通过确定抗氧化剂酶活性和小鼠中所选硒蛋白表达的变化来评估过量硒如何诱导氧化应激。对BALB / c小鼠（每组n ＝ 20）给予饮食，其中0.045（Se-边缘），0.1（Se-适量），0.4（Se-超营养）或0.8（Se-过量）mg Se / kg。通过实时定量逆转录-聚合酶链反应，从肝脏，肾脏和睾丸提取的RNA样品中定量基因表达。我们发现，饲喂少量或过量硒的小鼠肝脏中的谷胱甘肽过氧化物酶（GPx）和过氧化氢酶活性比硒充足组的小鼠降低。另外，与饲喂足够硒饮食的动物相比，仅在饲喂过量硒饮食的小鼠中超氧化物歧化酶和谷胱甘肽还原酶活性显着降低。硒超营养对GPx异构体1和4（GPx1和GPx4），GPx异构体3（GPx3）下调以及硒蛋白W（SelW）mRNA表达上调对肝mRNA水平没有影响。过量的硒饮食导致GPx1，GPx3和GPx4的肝mRNA水平降低，但GPx1，GPx3或SelW的睾丸mRNA水平没有变化。饮食硒对GPx4的睾丸mRNA水平没有影响。因此，我们的结果表明，硒的暴露会降低肝脏的抗氧化能力并引起肝功能障碍。发现膳食硒会根据暴露程度差异调节GPx家族或SelW的mRNA水平。因此，这些基因可能在与硒有关的毒性中起作用。

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