Leucine-2-alanien enkephalininduced δ opioid receptors internalization expressed stably in CHO cells

Wang Chun-He; ZHou De-He; Chen Jie

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Leucine-2-alanien enkephalininduced δ opioid receptors internalization expressed stably in CHO cells

机译：亮氨酸-2-丙氨酸脑啡肽诱导的δ阿片受体内在化在CHO细胞中稳定表达

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AIM: To characterize the internalization of δ opioid receptors (DOR) stably expressed in Chinese hamster ovary (CHO) cells and the role of the C-terminal in this proces. METHODS: Receptor membrane anchoring was shown by immunofluorescence micro- scopy. Receptor internalization was assessed by measuring the radioligand binding resistant to the acid- buffer wash. CONCLUSION: DOR internalization Is an agonist and clathrin-coated pits dependent, but Post-receptor cellular signal transduction independent Process; moreover, the C-terminal of DOR, not Engaged in membrane anchoring, affects the Initialization of DOR internalization.

机译：目的：描述稳定在中国仓鼠卵巢（CHO）细胞中表达的δ阿片受体（DOR）的内在化及其在此过程中C末端的作用。方法：通过免疫荧光显微镜观察受体膜的锚定。通过测量对酸缓冲液洗涤的抗放射性配体结合来评估受体内化。结论：DOR内在化是激动剂和网格蛋白包被的凹坑依赖性的，但是受体后细胞信号转导的独立过程。此外，DOR的C末端（未参与膜锚定）会影响DOR内部化的初始化。

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《Acta Pharmacologica Sinica》 |1999年第6期|p.491-494|共4页
作者
Wang Chun-He; ZHou De-He; Chen Jie;
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正文语种 eng
中图分类药理学;
关键词
leucine-2-alanine enkephalin; CHO cells; radioligand assay;

机译：亮氨酸-2-丙氨酸脑啡肽;CHO细胞;放射性配体测定;

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2. Agonist-induced receptor internalization in Chinese hamster ovary cells stably co-expressing β1- and β2-adrenergic receptors [J] . Yoshihara T., Yonoki Y., Saito M., Biological & pharmaceutical bulletin . 2013,第1期

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3. Functional coupling, desensitization and internalization of virally expressed micro opioid receptors in cultured dorsal root ganglion neurons from micro opioid receptor knockout mice. [J] . Walwyn WM, Keith DE, Wei W, Neuroscience: An International Journal under the Editorial Direction of IBRO . 2004,第1期

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Leucine-2-alanien enkephalininduced δ opioid receptors internalization expressed stably in CHO cells

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