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首页> 外文期刊>Advance journal of food science and technology >Antioxidant Activity in vitro and in vivo of Auricularia auricula Polysaccharides through Different Extraction Processes
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Antioxidant Activity in vitro and in vivo of Auricularia auricula Polysaccharides through Different Extraction Processes

机译：不同提取工艺对木耳多糖体外和体内抗氧化活性的影响

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摘要

In this study, antioxidant effects in vitro and in vivo by the Caenorhabditis elegans model of Auriculaia auricula polysaccharides (AAP1, AAP2 and AAP3) were used to compare these three kinds of polysaccharide extraction processes (hot water extraction, enzyme extraction and alkali extraction). And the monosaccharide composition of these polysaccharides was determined by Gas Chromatography (GC) method. Results suggested that AAP2, which was mainly composed of arabinose, D-xylose, 2-Deoxy-D-arabino-hexose, mannose, glucose and galactose with the molar ratio of 1:0.44:0.33:1.67:1:0.17, possessed stronger antioxidant activity. It could scavenge free radicals, up-regulation of antioxidant enzyme (superoxide dismutase (SOD) by 91.25% and catalase (CAT) by 36.67%, compared with control). It could also reduce the level of Reactive Oxygen Species (ROS) in C. elegans under oxidative stress. In conclusion, enzyme extraction is better in preparing AAP.
机译：在这项研究中，通过金合欢多糖（AAP1，AAP2和AAP3）的秀丽隐杆线虫模型在体内和体外的抗氧化作用被用来比较这三种多糖的提取过程（热水提取，酶提取和碱提取）。然后通过气相色谱法（GC）测定这些多糖的单糖组成。结果表明，AAP2主要由阿拉伯糖，D-木糖，2-脱氧-D-阿拉伯糖基己糖，甘露糖，葡萄糖和半乳糖组成，摩尔比为1：0.44：0.33：1.67：1：0.17。抗氧化活性。与对照相比，它可以清除自由基，上调抗氧化酶（超氧化物歧化酶（SOD）达91.25％，过氧化氢酶（CAT）达36.67％）。它也可以降低线虫在氧化胁迫下的活性氧（ROS）水平。总之，酶提取在制备AAP中更好。

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