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首页> 外文期刊>Advanced Functional Materials >Interaction of Human Mesenchymal Stem Cells with Soft Nanocomposite Hydrogels Based on Polyethylene Glycol and Dendritic Polyglycerol
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Interaction of Human Mesenchymal Stem Cells with Soft Nanocomposite Hydrogels Based on Polyethylene Glycol and Dendritic Polyglycerol

机译：人间充质干细胞与基于聚乙二醇和树突状聚甘油的软纳米复合水凝胶的相互作用

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摘要

Keeping the stemness of human mesenchymal stem cells (hMSCs) and their adipocyte differentiation potential is critical for clinical use. However, these features are lost on traditional substrates. hMSCs have often been studied on stiff materials whereas culturing hMSCs in their native niche increases their potential. Herein, a patterned hydrogel nanocomposite with the stiffness of liver tissues is obtained without any molding process. To investigate hMSCs' mechanoresponse to the material, the RGD spacing units and the stiffness of the hydrogels are dually tuned via the linker length. This work suggests that hMSCs' locomotion is influenced by the nature of the hydrogel layer (bulk or thin film). Contrary to on bulk surfaces, cell traction occurs during cell spreading on thin films. In addition, hMSCs' spreading behavior varies from shorter to longer linker-based hydrogels, where on both surfaces hMSCs maintains their stemness as well as their adipogenic differentiation potential with a higher number of adipocytes for nanocomposites with a longer polymer linker. Overall, this work addresses the need for a new alternative for hMSCs culture allowing the cells to differentiate exclusively into adipocytes. This material represents a cell-responsive platform with a tissue-mimicking architecture given by the mechanical and morphological properties of the hydrogel.
机译：保持人间充质干细胞（hMSCs）的干性及其脂肪细胞分化潜能对于临床应用至关重要。然而，这些特征在传统基板上丢失了。人类间充质干细胞经常在硬质材料上进行研究，而在其原生生态位中培养人类间充质干细胞则增加了其潜力。在此，无需任何模制过程即可获得具有肝组织刚度的图案化水凝胶纳米复合材料。为了研究hMSC对材料的机械响应，RGD间隔单位和水凝胶的刚度通过接头长度进行了双重调节。这项工作表明，hMSCs的运动受水凝胶层（本体或薄膜）的性质影响。与大块表面相反，在薄膜上铺展细胞期间会发生细胞牵引。此外，hMSCs的扩散行为从较短的接头到更长的基于接头的水凝胶不等，其中在hMSCs的两个表面上都保持了其干性和成脂分化潜能，而对于具有更长的聚合物接头的纳米复合材料而言，hMSCs的脂肪细胞数量更多。总的来说，这项工作满足了对hMSCs培养的新选择的需要，该选择允许细胞仅分化为脂肪细胞。该材料代表具有细胞模仿平台的细胞响应平台，该平台由水凝胶的机械和形态学特性决定。

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