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首页> 外文期刊>Advanced Functional Materials >Nanogap-Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection: Application to Gastric and Breast Cancer Tissues Diagnosis
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Nanogap-Rich Au Nanowire SERS Sensor for Ultrasensitive Telomerase Activity Detection: Application to Gastric and Breast Cancer Tissues Diagnosis

机译：适用于超敏端粒酶活性检测的富含Nanogap的Au Nanowire SERS传感器：在胃和乳腺癌组织诊断中的应用

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摘要

Telomerase has attracted much attention as a universal cancer biomarker because telomerase is overexpressed in more than 85% of human cancer cells while suppressed in normal somatic cells. Since a strong association exists between telomerase activity and human cancers, the development of effective telomerase activity assay is critically important. Here, a nanogap-rich Au nanowire (NW) surface-enhanced Raman scattering (SERS) sensor is reported for detection of telomerase activity in various cancer cells and tissues. The nanogap-rich Au NWs are constructed by deposition of nano-particles on single-crystalline Au NWs and provided highly reproducible SERS spectra. The telomeric substrate (TS) primer-attached nanogap-rich Au NWs can detect telomerase activity through SERS measurement after the elongation of TS primers, folding into G-quadruplex structures, and intercalation of methylene blue. This sensor enables us to detect telomerase activity from various cancer cell lines with a detection limit of 0.2 cancer cells mL(-1). Importantly, the nanogap-rich Au NW sensor can diagnose gastric and breast cancer tissues accurately. The nanogap-rich Au NW sensors show strong SERS signals only in the presence of tumor tissues excised from 16 tumor-bearing mice, while negligible signals in the presence of heated tumor tissues or normal tissues. It is anticipated that nanogap-rich Au NW SERS sensors can be used for a universal cancer diagnosis and further biomedical applications including a diverse biomarker sensing.
机译：端粒酶作为一种普遍的癌症生物标记物已经引起了广泛的关注，因为端粒酶在超过85％的人类癌细胞中过表达，而在正常的体细胞中则受到抑制。由于端粒酶活性与人类癌症之间存在密切联系，因此开发有效的端粒酶活性测定至关重要。在此，报道了一种富含纳米间隙的金纳米线（NW）表面增强拉曼散射（SERS）传感器，用于检测各种癌细胞和组织中的端粒酶活性。富纳米间隙的金纳米线是通过在单晶金纳米线上沉积纳米颗粒而构建的，并提供了高度可再现的SERS光谱。 TS引物延伸后，折叠成G-四链体结构，并插入亚甲基蓝后，端粒底物（TS）上附着有纳米间隙的富Au NW可以通过SERS测量来检测端粒酶活性。该传感器使我们能够检测各种癌细胞系中的端粒酶活性，检测限为0.2个癌细胞mL（-1）。重要的是，富含纳米间隙的金纳米线传感器可以准确诊断胃癌和乳腺癌组织。仅在从16只荷瘤小鼠中切除的肿瘤组织存在下，富含纳米间隙的Au NW传感器才会显示强SERS信号，而在存在加热的肿瘤组织或正常组织的情况下可以忽略不计。可以预期，富含纳米间隙的Au NW SERS传感器可用于普遍的癌症诊断和进一步的生物医学应用，包括多种生物标志物传感。

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  • 来源
    《Advanced Functional Materials》 |2017年第37期|1701832.1-1701832.10|共10页
  • 作者

    Eom Gayoung; Kim Hongki; Hwang Ahreum; Son Hye-Young; Choi Yuna; Moon Jeong; Kim Donghyeong; Lee Miyeon; Lim Eun-Kyung; Jeong Jinyoung; Huh Yong-Min; Seo Min-Kyo; Kang Taejoon; Kim Bongsoo;

  • 作者单位

    Korea Adv Inst Sci & Technol, Dept Chem, Daejeon 34141, South Korea|KRIBB, Hazards Monitoring Bionano Res Ctr, Daejeon 34141, South Korea;

    Korea Adv Inst Sci & Technol, Dept Chem, Daejeon 34141, South Korea;

    Korea Adv Inst Sci & Technol, Dept Chem, Daejeon 34141, South Korea|KRIBB, Hazards Monitoring Bionano Res Ctr, Daejeon 34141, South Korea;

    Yonsei Univ, Dept Radiol, Seoul 03772, South Korea|Yonsei Univ, Severance Biomed Sci Inst, Seoul 03772, South Korea;

    Yonsei Univ, Dept Radiol, Seoul 03772, South Korea;

    KRIBB, Hazards Monitoring Bionano Res Ctr, Daejeon 34141, South Korea;

    Korea Adv Inst Sci & Technol, Dept Phys, Daejeon 34141, South Korea;

    Korea Adv Inst Sci & Technol, Dept Chem, Daejeon 34141, South Korea;

    KRIBB, Hazards Monitoring Bionano Res Ctr, Daejeon 34141, South Korea|KRIBB, BioNano Hlth Guard Res Ctr, Daejeon 34141, South Korea|UST, KRIBB Sch Biotechnol, Dept Nanobiotechnol, Daejeon 34113, South Korea;

    KRIBB, Hazards Monitoring Bionano Res Ctr, Daejeon 34141, South Korea|KRIBB, BioNano Hlth Guard Res Ctr, Daejeon 34141, South Korea|UST, KRIBB Sch Biotechnol, Dept Nanobiotechnol, Daejeon 34113, South Korea;

    Yonsei Univ, Dept Radiol, Seoul 03772, South Korea|Yonsei Univ, YUHS KRIBB Med Convergence Res Inst, Seoul 03772, South Korea;

    Korea Adv Inst Sci & Technol, Dept Phys, Daejeon 34141, South Korea;

    KRIBB, Hazards Monitoring Bionano Res Ctr, Daejeon 34141, South Korea|KRIBB, BioNano Hlth Guard Res Ctr, Daejeon 34141, South Korea|UST, KRIBB Sch Biotechnol, Dept Nanobiotechnol, Daejeon 34113, South Korea;

    Korea Adv Inst Sci & Technol, Dept Chem, Daejeon 34141, South Korea;

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  • 正文语种 eng
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  • 关键词

    gold nanowires; nanogaps; sensors; surface-enhanced Raman scattering; telomerase activity;

    机译：金纳米线;纳米粒子;传感器;表面增强拉曼散射;端粒酶活性;

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