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首页> 外文期刊>Advanced Functional Materials >Design of Multistimuli Responsive Hydrogels Using Integrated Modeling and Genetically Engineered Silk-Elastin-Like Proteins
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Design of Multistimuli Responsive Hydrogels Using Integrated Modeling and Genetically Engineered Silk-Elastin-Like Proteins

机译：使用集成建模和基因工程的真丝-弹性蛋白样蛋白设计多刺激响应水凝胶。

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摘要

Elastomeric, robust, and biocompatible hydrogels are rare, while the need for these types of biomaterials in biomedical-related uses remains high. Here, a new family of genetically engineered silk-elastin-like proteins (SELPs) with encoded enzymatic crosslinking sites is developed for a new generation of stimuli-responsive yet robust hydrogels. Input into the designs is guided by simulation and realized via genetic engineering strategies. The avoidance of gamma irradiation or chemical crosslinking during gel fabrication, in lieu of an enzymatic process, expands the versatility of these new gels for the incorporation of labile proteins and cells. In the present study, the new SELP hydrogels offer sequence-dependent, reversible stimuli-responsive features. Their stiffness covers almost the full range of the elasticity of soft tissues. Further, physical modification of the silk domains provides a secondary control point to fine-tune mechanical stiffness while preserving stimuli-responsive features, with implications for a variety of biomedical material and device needs.
机译：弹性，坚固且具有生物相容性的水凝胶很少见，而在生物医学相关用途中对这类生物材料的需求仍然很高。在这里，开发了具有编码的酶交联位点的遗传工程化的新型丝绸-弹性蛋白样蛋白（SELP）家族，用于新一代的刺激应答性强健的水凝胶。通过模拟指导设计的输入，并通过基因工程策略实现。代替酶促过程，避免在凝胶制造过程中进行伽马射线辐照或化学交联，可以扩大这些新凝胶用于不稳定蛋白质和细胞掺入的多功能性。在本研究中，新型SELP水凝胶具有序列依赖性，可逆的刺激响应特性。它们的刚度几乎覆盖了软组织的所有弹性。此外，丝域的物理修饰提供了一个辅助控制点，可在保持刺激响应特征的同时微调机械刚度，这对各种生物医学材料和装置的需求都有影响。

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  • 来源
    《Advanced Functional Materials》 |2016年第23期|4113-4123|共11页
  • 作者

    Huang Wenwen; Tarakanova Anna; Dinjaski Nina; Wang Qin; Xia Xiaoxia; Chen Ying; Wong Joyce Y.; Buehler Markus J.; Kaplan David L.;

  • 作者单位

    Tufts Univ, Dept Biomed Engn, 4 Colby St, Medford, MA 02155 USA;

    MIT, Dept Civil & Environm Engn, LAMM, 77 Massachusetts Ave, Cambridge, MA 02139 USA;

    Tufts Univ, Dept Biomed Engn, 4 Colby St, Medford, MA 02155 USA;

    Tufts Univ, Dept Biomed Engn, 4 Colby St, Medford, MA 02155 USA|Hubei Univ, Coll Life Sci, Hubei Collaborat Innovat Ctr Green Transformat Bi, Wuhan 430062, Peoples R China;

    Tufts Univ, Dept Biomed Engn, 4 Colby St, Medford, MA 02155 USA|Shanghai Jiao Tong Univ, Sch Life Sci & Biotechnol, State Key Lab Microbial Metab, 800 Dong Chuan Rd, Shanghai 200240, Peoples R China;

    Tufts Univ, Dept Biomed Engn, 4 Colby St, Medford, MA 02155 USA;

    Boston Univ, Dept Biomed Engn, Boston, MA 02215 USA|Boston Univ, Dept Mat Sci & Engn, Boston, MA 02215 USA;

    MIT, Dept Civil & Environm Engn, LAMM, 77 Massachusetts Ave, Cambridge, MA 02139 USA;

    Tufts Univ, Dept Biomed Engn, 4 Colby St, Medford, MA 02155 USA;

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