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Functional Patterning of Biopolymer Thin Films Using Enzymes and Lithographic Methods

机译：使用酶和光刻方法对生物聚合物薄膜进行功能性图案化

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Two different lithographic techniques for the patterning of thin biopolymer films are developed. The first method is based on using a microstructured elastomeric mold for the structuring of thin films of regenerated cellulose. The thin films are manufactured by spin-coating of trimethylsilyl cellulose (TMSC) and subsequent regeneration. The microchannels formed by the mold and the cellulose film are filled with a cellulase solution by capillary action. In the areas exposed to the enzyme solution, the cellulose film is digested, whereas the area in contact with the mold is protected from the enzymatic activity. Optical thickness measurements, atomic force microscopy and fluorescent staining confirm a successful patterning of cellulose on several substrates by this method. The second method is based on the structured regeneration of thin TMSC films. TMSC surfaces are protected with metal masks and exposed to vapors of hydrochloric acid. These treatments result in hydrophilic cellulose structures surrounded by hydrophobic TMSC with differing physicochemical properties. Treatments of the obtained structures with cellulases allow the selective removal of pure cellulose, whereas a TMSC pattern remains on the surface. These TMSC can be regenerated back to pure cellulose by treatments with vapors of hydrochloric acid. The developed methods allow the effective fabrication of micropatterned biopolymer thin films suitable for further functionaliza-tion and application in, e.g., bioanalytical devices. This is shown by the immobilization and detection of single-stranded DNA on structured cellulose surfaces. Owing to the versatility of both patterning approaches the methods can be further extended to other combinations of substrates and enzymes.
机译：开发了两种不同的用于对生物聚合物薄膜进行构图的光刻技术。第一种方法是基于使用微结构的弹性体模具来构造再生纤维素的薄膜。通过三甲基甲硅烷基纤维素（TMSC）的旋涂和随后的再生来制造薄膜。由模具和纤维素膜形成的微通道通过毛细管作用被纤维素酶溶液填充。在暴露于酶溶液的区域中，纤维素膜被消化，而与霉菌接触的区域被保护免受酶活性的影响。光学厚度测量，原子力显微镜和荧光染色证实了通过这种方法可以成功地在几种基材上对纤维素进行构图。第二种方法是基于TMSC薄膜的结构化再生。 TMSC表面用金属掩膜保护，并暴露于盐酸蒸气中。这些处理导致亲水性纤维素结构被具有不同理化性质的疏水性TMSC包围。用纤维素酶处理所获得的结构允许选择性地除去纯纤维素，而在表面上保留了TMSC图案。这些TMSC可以通过用盐酸蒸气处理而再生为纯纤维素。所开发的方法允许有效地制造适合于进一步功能化并应用于例如生物分析装置中的微图案化的生物聚合物薄膜。通过在结构化纤维素表面上固定和检测单链DNA可以证明这一点。由于两种构图方法的通用性，该方法可以进一步扩展到底物和酶的其他组合。

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  • 来源
    《Advanced Functional Materials》 |2013年第3期|308-315|共8页
  • 作者

    Rupert Kargl; Tamilselvan Mohan; Stefan Koestler; Stefan Spirk; Ales Doliska; Karin Stana-Kleinschek; Volker Ribitsch;

  • 作者单位

    Karl-Franzens-University Graz Institute of Chemistry Heinrichstrage 28/111, A-8010 Graz, Austria;

    University of Maribor Laboratory for Characterization and Processing of Polymers Smetanova Ulica 17, 2000 Maribor, Slovenia;

    Joanneum Research MATERIALS-lnstitute for Surface Technologies and Photonics Steyrergasse 1 7, A-8010 Graz, Austria;

    University of Maribor Laboratory for Characterization and Processing of Polymers Smetanova Ulica 17, 2000 Maribor, Slovenia;

    University of Maribor Laboratory for Characterization and Processing of Polymers Smetanova Ulica 17, 2000 Maribor, Slovenia;

    University of Maribor Laboratory for Characterization and Processing of Polymers Smetanova Ulica 17, 2000 Maribor, Slovenia;

    Karl-Franzens-University Graz Institute of Chemistry Heinrichstrage 28/111, A-8010 Graz, Austria,Joanneum Research MATERIALS-lnstitute for Surface Technologies and Photonics Steyrergasse 1 7, A-8010 Graz, Austria;

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