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首页> 外文期刊>Advanced Materials >A Cyclometalated Iridium (Ⅲ) Complex as a Microtubule Probe for Correlative Super-Resolution Fluorescence and Electron Microscopy
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A Cyclometalated Iridium (Ⅲ) Complex as a Microtubule Probe for Correlative Super-Resolution Fluorescence and Electron Microscopy

机译：一种环状氧化铱（Ⅲ）复合物作为用于相关超分辨率荧光和电子显微镜的微管探针

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摘要

The visualization of microtubules by combining optical and electron microscopy techniques provides valuable information to understand correlated intracellular activities. However, the lack of appropriate probes to bridge both microscopic resolutions restricts the areas and structures that can be comprehended within such highly assembled structures. Here, a versatile cyclometalated iridium (III) complex is designed that achieves synchronous fluorescence-electron microscopy correlation. The selective insertion of the probe into a microtubule triggers remarkable fluorescence enhancement and promising electron contrast. The long-life, highly photostable probe allows live-cell super-resolution imaging of tubulin localization and motion with a resolution of approximate to 30 nm. Furthermore, correlative light-electron microscopy and energy-filtered transmission electron microscopy reveal the well-associated optical and electron signal at a high specificity, with an interspace of approximate to 41 angstrom of microtubule monomer in cells.
机译：通过组合光学和电子显微镜技术的微管可视化提供有价值的信息，以了解相关的细胞内活动。然而，缺乏适当的探头来桥接微观分辨率限制了可以在这种高度组装的结构内理解的区域和结构。这里，设计了一种多功能的环状氧化铱（III）复合物，其实现了同步荧光 - 电子显微镜相关性。将探针的选择性插入微管触发荧光增强和有前途的电子对比度。长寿命，高度光稳定的探针允许微管素定位的实心细胞超分辨率成像和运动的分辨率近似为30nm。此外，相关的光 - 电子显微镜和能量过滤的透射电子显微镜显微镜以高特异性揭示相关的光学和电子信号，其间隙在细胞中近似于41埃的微管单体。

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  • 来源
    《Advanced Materials》 |2020年第39期|2003901.1-2003901.7|共7页
  • 作者

    Tian Xiaohe; De Pace Cesare; Ruiz-Perez Lorena; Chen Bo; Su Rina; Zhang Mingzhu; Zhang Ruilong; Zhang Qiong; Wang Qin; Zhou Hongping; Wu Jieying; Zhang Zhongping; Tian Yupeng; Battaglia Giuseppe;

  • 作者单位

    Anhui Univ Sch Life Sci Hefei 230000 Peoples R China|Anhui Univ Dept Chem Hefei 230000 Peoples R China|Anhui Univ Inst Phys Sci & Informat Technol Hefei 230000 Peoples R China|UCL Dept Chem London WC1H 0AJ England;

    UCL Dept Chem London WC1H 0AJ England|UCL Inst Phys Living Syst London WC1E 6BT England|UCL EPSRC JEOL Ctr Liquid Phase Electron Microscopy London WC1H 0AJ England;

    UCL Dept Chem London WC1H 0AJ England|UCL Inst Phys Living Syst London WC1E 6BT England|UCL EPSRC JEOL Ctr Liquid Phase Electron Microscopy London WC1H 0AJ England;

    Tongji Univ Sch Mat Sci & Engn Shanghai 201804 Peoples R China;

    Anhui Univ Sch Life Sci Hefei 230000 Peoples R China;

    Anhui Univ Dept Chem Hefei 230000 Peoples R China;

    Anhui Univ Dept Chem Hefei 230000 Peoples R China;

    Anhui Univ Dept Chem Hefei 230000 Peoples R China;

    Anhui Agr Univ Biotechnol Ctr Hefei 230036 Peoples R China;

    Anhui Univ Dept Chem Hefei 230000 Peoples R China;

    Anhui Univ Dept Chem Hefei 230000 Peoples R China;

    Anhui Univ Dept Chem Hefei 230000 Peoples R China|Anhui Univ Inst Phys Sci & Informat Technol Hefei 230000 Peoples R China|Chinese Acad Sci CAS Ctr Excellence Nanosci Inst Intelligent Machines Hefei 230031 Peoples R China;

    Anhui Univ Dept Chem Hefei 230000 Peoples R China;

    Anhui Univ Dept Chem Hefei 230000 Peoples R China|Anhui Univ Inst Phys Sci & Informat Technol Hefei 230000 Peoples R China|UCL Inst Phys Living Syst London WC1E 6BT England|UCL EPSRC JEOL Ctr Liquid Phase Electron Microscopy London WC1H 0AJ England|Barcelona Inst Sci & Technol BIST Inst Bioengn Catalonia Barcelona 08007 Spain|Catalan Inst Res & Adv Studies Barcelona 08010 Spain;

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  • 正文语种 eng
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  • 关键词

    correlation light-electron microscopy; microtubules; organometallic probes; super-resolution microscopy;

    机译：相关光 - 电子显微镜;微管;有机金属探头;超分辨率显微镜;

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