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首页> 外文期刊>Aerobiologia >A study on the allergenicity and ontogeny of Acacia farnesiana pollen grains in guinea pigs
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A study on the allergenicity and ontogeny of Acacia farnesiana pollen grains in guinea pigs

机译：豚鼠相思粉花粉粒的致敏性和遗传的研究。

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摘要

Pollen grains as the angiosperm male gametophytes transfer male genetic material during sexual reproduction. Pollen grains are among the important plant allergens, such that almost 80-90 % of plant allergens are of pollen origin. Acacia farnesiana is a plant with economical values, and due to its resistance to dry climates, it has had a widespread distribution in Southern Iran. This study was aimed at investigating the allergenicity and the ontogeny of pollen grains of A. farnesiana. Pollen grains were collected from the blossoms and flowers of A. farnesiana in the suburbs of Ahvaz-Iran. Pollen extracts (15 %) were prepared in PBS (pH 7.2). 4-6-week-old male guinea pigs (Hartly) were used for allergenicity tests. Skin tests showed a significant increase in flare diameter. Clinical tests also showed a significant change in the levels of eosinophils, neu-trophils, and IgE. Histotechnical analysis was performed on male gametophytes, and photographs were taken using a camera-equipped light microscope. Pollen characteristics were identified using both light and electron microscopy. Sporoderm ultrastructure and pollen morphology were studied using Scanning Electron Microscopy. PAGE analysis of the total protein content of mature pollen grains showed seven clear bands of 10-83 kDa.
机译：花粉粒作为被子植物雄配子体在有性生殖过程中转移雄性遗传物质。花粉粒是重要的植物过敏原之一，因此几乎80-90％的植物过敏原是花粉来源。金合欢属植物具有经济价值，由于其对干旱气候的抵抗力，已在伊朗南部广泛分布。这项研究的目的是调查A. farnesiana花粉粒的致敏性和个体发育。花粉谷粒是从阿瓦士-伊朗郊区的法氏酵母的花朵中采集的。在PBS（pH 7.2）中制备花粉提取物（15％）。使用4-6周龄的雄性豚鼠（Hartly）进行致敏性测试。皮肤测试显示，耀斑直径显着增加。临床测试还显示，嗜酸性粒细胞，中性粒细胞和IgE的水平发生了显着变化。对雄配子体进行了组织技术分析，并使用配备相机的光学显微镜拍摄了照片。使用光学和电子显微镜鉴定花粉特征。使用扫描电子显微镜研究孢子的超微结构和花粉形态。对成熟花粉粒的总蛋白质含量的PAGE分析显示七个清晰的条带，为10-83 kDa。

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