• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Aerobiologia >The effects of air pollution on structures, proteins and allergenicity of pollen grains
【24h】

The effects of air pollution on structures, proteins and allergenicity of pollen grains

机译：空气污染对花粉粒结构，蛋白质和致敏性的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The prevalence of allergic disease has increased world wide during the last decades. Pollen allergy is the most typical form of allergic disease. The increase in its frequency during recent years is the most evident. Environmental factors play an important role in the problem of pollen allergy in large cities. The aim of this research is determination of allergenicity of Canna pollen in polluted and non-polluted conditions, detection of their allergenic proteins and also elucidation of some microscopic effects of air pollutants on pollen structure and proteins. Mature and immature pollen grains of Canna indica were collected from polluted and non-polluted areas. Pollen grains were studied by scanning electron microscopy. Mice were sensitized by injection of pollen extract and an adjuvant for five times. Allergy potency of different pollen extracts were compared by means of skin test, blood eosinophills number and IgE levels in sensitized and treated animals. Pollen proteins were studied by SDS-PGE and allergenic proteins were detected by immunoblotting techniques. Scanning electron microscope study of the pollen grains showed that in polluted areas, air born particles accumulated on the surface of pollen and changed both pollen's shape and pollen's tectum. Also many vesicles were released out of polluted pollen and the pollen material agglomerated on the surface of pollen. SDS-PAGE showed that different proteins exist in mature and immature pollen. In pollen collected from polluted area, some of protein bands between 22 and 45 kDa were disappeared . Also in all polluted pollen grains, protein content of pollen decreased in response to air pollution causing the release of pollen proteins. According to our experiments and regarding induction of allergic symptoms, the polluted pollen is more effective than non-polluted one, and mature pollen has more allergy potency than immature one.
机译：在过去的几十年中，世界范围内过敏性疾病的患病率呈上升趋势。花粉过敏是过敏性疾病的最典型形式。近年来频率的增加最为明显。在大城市中，环境因素在花粉过敏问题中起着重要作用。这项研究的目的是确定Canna花粉在污染和非污染条件下的致敏性，检测其致敏蛋白，并阐明空气污染物对花粉结构和蛋白的微观影响。从污染区和非污染区收集印度成熟的和未成熟的花粉粒。通过扫描电子显微镜研究花粉粒。通过注射花粉提取物和佐剂五次使小鼠致敏。通过皮肤试验，血液嗜酸性粒细胞数和IgE水平，在致敏和治疗动物中比较了不同花粉提取物的过敏效力。通过SDS-PGE研究花粉蛋白，并通过免疫印迹技术检测致敏蛋白。扫描电子显微镜对花粉粒的研究表明，在受污染的地区，空气中的悬浮颗粒聚集在花粉表面，并改变了花粉的形状和花粉的构造。还从污染的花粉中释放出许多囊泡，并且花粉物质聚集在花粉表面上。 SDS-PAGE表明成熟和未成熟花粉中存在不同的蛋白质。在从污染区收集的花粉中，22至45 kDa之间的某些蛋白带消失了。同样在所有被污染的花粉粒中，花粉的蛋白质含量响应于空气污染而降低，从而导致花粉蛋白质的释放。根据我们的实验并考虑到过敏症状的诱导，受污染的花粉比未受污染的花粉更有效，而成熟的花粉比未成熟的花粉更有效。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号