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首页> 外文期刊>Aerobiologia >Detection of E. coli DH5alpha specific bacteriophage in the vicinity of a water treatment plant
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Detection of E. coli DH5alpha specific bacteriophage in the vicinity of a water treatment plant

机译：在水处理厂附近检测大肠杆菌DH5alpha特异性噬菌体

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摘要

Air in the vicinity of a water treatment plant scheduled for redevelopment was monitored for aerosols containing a bacteriophage specific for the Escherichia coli (E. coli) strain DH5alpha bacterium. An assay for the detection of the specific airborne bacteriophage was developed. Sampling was carried out prior to and after completion of development work to the plant. Preliminary experiments showed water from the plant contains the bacteriophage specific for the E. coli DH5alpha bacterium. Two field studies were completed before work on the treatment plant began. The first study, conducted under wet and windy conditions, used only passive air sampling to monitor air at 3 selected locations adjacent to the plant. E. coli DH5alpha specific bacteriophage was positively detected, albeit at low levels. However, both the passive and a new direct air sampling technique were employed in the second field study, conducted under dry and windy conditions. In this instance, neither method detected specific bacteriophage. Further work was undertaken 6 months after modifications to the plant had been completed. Passive and direct air sampling techniques were again employed at each of the same 3 selected locations. Samples from both of these methods contained no bacteriophage. As a check, the samples were also analysed using an alternative procedure. No bacteriophage was detected. The results generated indicate that the levels of airborne specific bacteriophage in the vicinity of the plant appear to be extremely low, and the direct air sampling technique successful.
机译：监测计划重新开发的水处理厂附近的空气中的气溶胶，其中包含对大肠杆菌（E. coli）菌株DH5alpha细菌具有特异性的噬菌体。开发了一种检测特定的空中噬菌体的方法。在工厂开发工作完成之前和之后进行采样。初步实验表明，来自植物的水含有对大肠杆菌DH5alpha细菌具有特异性的噬菌体。在处理厂开始工作之前，已经完成了两项现场研究。在潮湿多风的条件下进行的第一项研究仅使用被动空气采样来监测与工厂相邻的3个选定位置的空气。阳性检测到大肠杆菌DH5alpha特异性噬菌体，尽管其水平较低。然而，在干燥和有风的条件下进行的第二次野外研究均采用了被动和新的直接空气采样技术。在这种情况下，两种方法均未检测到特定的噬菌体。对工厂的改造完成后的6个月，进行了进一步的工作。在相同的三个选定位置中的每个位置，再次采用了被动和直接空气采样技术。这两种方法的样品均不含噬菌体。作为检查，还使用替代程序分析了样品。未检测到噬菌体。产生的结果表明，植物附近的空气传播特异性噬菌体水平似乎极低，直接空气采样技术取得了成功。

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