Usefulness of using three different culture media for mold recovery in exposure assessment studies

Caroline Duchaine; Anne Meriaux; Paul Comtois

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Usefulness of using three different culture media for mold recovery in exposure assessment studies

机译：在暴露评估研究中使用三种不同培养基进行霉菌恢复的有用性

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The choice of culture media when performing exposure assessment for mold quantification is difficult since no standard methods exist and, in many cases, there is no way to predict, before hand, the mold population present and thus, select the most appropriate media. The aim of this project was to compare the efficiency and usefulness of 3 widely used culture media during the recovery of molds in sawmills. Andersen microbial samplers (AMS) and all-glass impingers-30 (AGI-30) were used to sample 51 work sites within 17 sawmills. Rose Bengal Agar (RBA), Czapek Solution Agar (CZA) and Sabouraud Dextrose Agar (SDA) were used directly in AMS and to plate AGI-liquid. The results show that there was no signification difference between the three culture media concerning the counts and the species recovery. However, SDA often lead to non usable counts. The variations obtained with the different culture media when AGI-30 were plated in triplicate was also not significantly different. However, only 19% of the species recovered at one site were present on all culture media. We believe the use of various media increases the chances of obtaining valid counts and an adequate species recovery.

机译：在进行暴露评估以进行霉菌定量时，很难选择培养基，因为不存在标准方法，而且在许多情况下，无法事先预测存在的霉菌数量，因此无法选择最合适的培养基。该项目的目的是比较锯木厂霉菌回收过程中3种广泛使用的培养基的效率和实用性。使用安徒生微生物取样器（AMS）和全玻璃撞击器30（AGI-30）在17个锯木厂的51个工作地点取样。玫瑰孟加拉琼脂（RBA），Czapek溶液琼脂（CZA）和Sabouraud右旋糖琼脂（SDA）直接用于AMS中并涂AGI液体。结果表明，三种培养基在数量和物种恢复方面没有显着差异。但是，SDA通常会导致无法使用的计数。将AGI-30一式三份铺板时，使用不同培养基获得的变异也没有显着差异。但是，在一个地点回收的物种中只有19％存在于所有培养基中。我们相信使用各种介质会增加获得有效计数和适当物种恢复的机会。

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《Aerobiologia》 |2002年第4期|p.245-251|共7页
作者
Caroline Duchaine; Anne Meriaux; Paul Comtois;
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Departement de Biochimie-Microbiologie, Universite Laval, Quebec, Canada;

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正文语种 eng
中图分类生物科学;
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1. Enhanced recovery of Arcobacter spp. using NaCl in culture media and re-assessment of the traits of Arcobacter marinus and Arcobacter halophilus isolated from marine water and shellfish [J] . Nuria Salas-Masso, Karl B. Andree, M. Dolors Furones, The Science of the Total Environment . 2016,第octa1期

机译：增强了Arcobacter spp的回收率。在培养基中使用NaCl并重新评估从海水和贝类中分离出的海藻杆菌和嗜盐杆菌的特征
2. An Environmental Assessment of Mold Concentrations and Potential Mycotoxin Exposures in the Greater Southeast Texas Area [J] . Noreen N. Khan, Bobby L. Wilson Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering . 2003,第12期

机译：德克萨斯州东南部地区霉菌浓度和霉菌毒素暴露的环境评估
3. Two decades of exposure assessment studies on chromate production waste in Jersey City, New Jersey - What we have learned about exposure characterization and its value to public health and remediation [J] . SternA.H., GochfeldM., LioyP.J. Journal of exposure science & environmental epidemiology . 2013,第1期

机译：新泽西州泽西市关于铬酸盐生产废料的暴露评估研究长达二十年-我们了解到的暴露特性及其对公共卫生和补救的价值
4. Home exposure to molds: quantitative PCR using the Environmental Relative Moldiness Index (ERMI) compared to fungal cultures in French dwellings [C] . Delphine Meheust, Tiina Reponen, Stephen Vesper, Annual conference of the International Society of Exposure Science . 2014

机译：家庭中霉菌的暴露：与法国住宅中的真菌培养相比，使用环境相对霉变指数（ERMI）进行定量PCR
5. An environmental assessment of mold concentrations and potential mycotoxin exposure in the greater southeast Texas area. [D] . Khan, Noreen N. 2003

机译：德克萨斯州东南部地区霉菌浓度和潜在霉菌毒素暴露的环境评估。
6. Procedures to assist healthcare providers to determine when home assessments for potential mold exposure are warranted [O] . Ginger L. Chew, W. Elliott Horner, Kevin Kennedy, -1

机译：协助医疗保健提供者确定何时需要对可能的霉菌暴露进行家庭评估的程序
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8. Recovery from Catastrophic Weather: Mold Exposure and Health-Related Training. [R] . Schenck, P., Buchanan, M., Burcher, J., 2016

机译：从灾难性天气中恢复：霉菌暴露和健康相关培训。

Usefulness of using three different culture media for mold recovery in exposure assessment studies

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